Abstract |
Huang-lian-jie-du-tang ( HLJDT), a traditional Chinese medicine, has been shown to improve insulin resistance (IR) induced by inflammation, a key event in the development of metabolic syndrome (MS). The present study aimed to investigate the protective effects of HLJDT on MS and explore the underlying mechanism. MS rats were established with obese-diets and treated with normal saline, aspirin or HLJDT. The myocardial lesions were identified by echocardiogram, transmission electron microscope, and Sirius-red staining. The inflammatory cytokines were measured by ELISA and real-time PCR. The activation of NF-κB, JNK, SOCS3, IRS1 and AKT in the heart was detected by immunohistochemistry and Western blot analysis. Compared with the controls, MS rats developed obvious obesity, hypertension, dyslipidemia, IR, inflammation, and cardiac damage. Moreover, phosphorylated IRS-1 at Ser307 was correlated with the activation of NF-κB, JNK and SOCS3 and the inhibition of AKT in the heart from MS rats. These data suggest that serine phosphorylation of IRS-1 in response to inflammation is mediated, in part, by NF-κB, JNK and SOCS3. Notably, HLJDT inhibited the activation of NF-κB and reduced serine phosphorylation of IRS-1. In summary, HLJDT protects myocardium from IR-mediated injury by inhibiting serine phosphorylation of IRS-1 in MS rats.
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Authors | Chuan Bao Li, Xiao Xing Li, Yu Guo Chen, Hai Qing Gao, Pei Li Bu, Yun Zhang, Xiao Ping Ji |
Journal | PloS one
(PLoS One)
Vol. 8
Issue 6
Pg. e67530
( 2013)
ISSN: 1932-6203 [Electronic] United States |
PMID | 23840732
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cardiotonic Agents
- Insulin Receptor Substrate Proteins
- Irs1 protein, rat
- NF-kappa B
- Socs3 protein, rat
- Suppressor of Cytokine Signaling 3 Protein
- Suppressor of Cytokine Signaling Proteins
- Intercellular Adhesion Molecule-1
- Collagen
- Proto-Oncogene Proteins c-akt
- JNK Mitogen-Activated Protein Kinases
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Topics |
- Animals
- Blood Pressure
(drug effects)
- Cardiotonic Agents
(pharmacology)
- Collagen
(metabolism)
- Down-Regulation
(drug effects)
- Heart
(drug effects, physiopathology)
- Heart Rate
(drug effects)
- Inflammation
(physiopathology)
- Insulin Receptor Substrate Proteins
(metabolism)
- Insulin Resistance
(physiology)
- Intercellular Adhesion Molecule-1
(metabolism)
- JNK Mitogen-Activated Protein Kinases
(metabolism)
- Male
- Medicine, Chinese Traditional
(methods)
- Metabolic Diseases
(complications)
- Myocytes, Cardiac
(drug effects, metabolism, physiology)
- NF-kappa B
(metabolism)
- Phosphorylation
(drug effects)
- Proto-Oncogene Proteins c-akt
(metabolism)
- Rats
- Rats, Wistar
- Suppressor of Cytokine Signaling 3 Protein
- Suppressor of Cytokine Signaling Proteins
(metabolism)
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