Validation-based insertional mutagenesis for identification of Nup214 as a host factor for EV71 replication in RD cells.

Abstract

Lentiviral validation-based insertional mutagenesis (VBIM) is a sophisticated, forward genetic approach that is used for the investigation of signal transduction in mammalian cells. Using VBIM, we conducted function-based genetic screening for host genes that affect enterovirus 71 (EV71) viral replication. This included host factors that are required for the life cycle of EV71 and host restriction factors that inhibit EV71 replication. Several cell clones, resistant to EV71, were produced using EV71 infection as a selection pressure and the nuclear pore protein 214 (Nup214) was identified as a host factor required for EV71 replication. In SD2-2, the corresponding VBIM lentivirus transformed clone, the expression of endogenous Nup214 was significantly down-regulated by the reverse inserted VBIM promoter. After Cre recombinase-mediated excision of the VBIM promoter, the expression of Nup214 recovered and the clone regained sensitivity to the EV71 infection. Furthermore, over-expression of Nup214 in the cells suggested that Nup214 was promoting EV71 replication. Results of this study indicate that a successful mutagenesis strategy has been established for screening host genes related to viral replication.

• Authors: Bei Wang, Xiaoyu Zhang, Zhendong Zhao
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 437 Issue 3 Pg. 452-6 (Aug 02 2013) ISSN: 1090-2104 [Electronic] United States
• PMID: 23831628 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Validation Study)
• Copyright: Copyright © 2013 Elsevier Inc. All rights reserved.

Chemical References

• NUP214 protein, human
• Nuclear Pore Complex Proteins

Topics

• Clone Cells
• Enterovirus A, Human (genetics, physiology)
• Enterovirus Infections (genetics, pathology, virology)
• Genetic Testing
• HEK293 Cells
• Humans
• Lentivirus (genetics)
• Mutagenesis, Insertional (methods)
• Nuclear Pore Complex Proteins (genetics)
• Promoter Regions, Genetic
• Rhabdomyosarcoma (genetics, virology)
• Tumor Cells, Cultured
• Virus Replication (genetics)