Abstract |
An ethanol-soluble pigment extract was separated from fermented Zijuan Pu-erh tea. The compositions of the ethanol soluble pigment extract were analyzed by high-performance liquid chromatography-tandem mass spectroscopy (HPLC-MS/MS). The extract was prepared into a series of ethanol solutions and analyzed for free radical-scavenging activities (against two free radicals: 1,1-diphenyl-2-picrylhydrazyl (DPPH) and (2,2,6,6-tetramethylpiperidin-1-yl)oxyl (TEMPO)) and in vitro anti-oxidative properties. Electron spin resonance spectroscopy showed that the peaks of DPPH and TEMPO decreased with increasing extract concentration, suggesting that the extract had excellent free radical-scavenging activities. In vitro cell culture suggested that, at 50-200 mg/L, the extract had no measurable effect on the viability of vascular endothelial cells (ECV340) but produced significant protective effects for cells that underwent oxidative injuries due to hydrogen peroxide (H₂O₂) treatment. Compared with the H₂O₂ treatment alone cells group, 200 mg/L of the extract increased the activity of superoxide dismutase (SOD) in cells by 397.3%, and decreased the concentration of malondialdehyde (MDA) and the activity of lactate acid dehydrogenase (LDH) by 47.8% and 69.6%, respectively. These results suggest that the extract has excellent free radical scavenging and anti-oxidative properties.
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Authors | Jiang Ping Fan, Chong Fan, Wen Min Dong, Bin Gao, Wei Yuan, Jia Shun Gong |
Journal | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
(Food Chem Toxicol)
Vol. 59
Pg. 527-33
(Sep 2013)
ISSN: 1873-6351 [Electronic] England |
PMID | 23831194
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2013 Elsevier Ltd. All rights reserved. |
Chemical References |
- Antioxidants
- Free Radical Scavengers
- Pigments, Biological
- Plant Extracts
- Solvents
- Tea
- Ethanol
- Malondialdehyde
- Lactate Dehydrogenases
- Superoxide Dismutase
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Topics |
- Antioxidants
(chemistry, isolation & purification, metabolism, pharmacology)
- Camellia sinensis
(chemistry, metabolism)
- Cell Line
- Cell Survival
(drug effects)
- China
- Diet
(ethnology)
- Endothelium, Vascular
(cytology, drug effects, enzymology, metabolism)
- Ethanol
(chemistry)
- Ethnopharmacology
- Fermentation
- Food Handling
- Free Radical Scavengers
(adverse effects, chemistry, isolation & purification, pharmacology)
- Humans
- Lactate Dehydrogenases
(chemistry, metabolism)
- Malondialdehyde
(antagonists & inhibitors, metabolism)
- Oxidative Stress
(drug effects)
- Pigments, Biological
(adverse effects, chemistry, isolation & purification, pharmacology)
- Plant Extracts
(adverse effects, chemistry, isolation & purification, pharmacology)
- Solubility
- Solvents
(chemistry)
- Superoxide Dismutase
(chemistry, metabolism)
- Tea
(chemistry, microbiology)
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