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Free radical scavenging and anti-oxidative activities of an ethanol-soluble pigment extract prepared from fermented Zijuan Pu-erh tea.

Abstract
An ethanol-soluble pigment extract was separated from fermented Zijuan Pu-erh tea. The compositions of the ethanol soluble pigment extract were analyzed by high-performance liquid chromatography-tandem mass spectroscopy (HPLC-MS/MS). The extract was prepared into a series of ethanol solutions and analyzed for free radical-scavenging activities (against two free radicals: 1,1-diphenyl-2-picrylhydrazyl (DPPH) and (2,2,6,6-tetramethylpiperidin-1-yl)oxyl (TEMPO)) and in vitro anti-oxidative properties. Electron spin resonance spectroscopy showed that the peaks of DPPH and TEMPO decreased with increasing extract concentration, suggesting that the extract had excellent free radical-scavenging activities. In vitro cell culture suggested that, at 50-200 mg/L, the extract had no measurable effect on the viability of vascular endothelial cells (ECV340) but produced significant protective effects for cells that underwent oxidative injuries due to hydrogen peroxide (H₂O₂) treatment. Compared with the H₂O₂ treatment alone cells group, 200 mg/L of the extract increased the activity of superoxide dismutase (SOD) in cells by 397.3%, and decreased the concentration of malondialdehyde (MDA) and the activity of lactate acid dehydrogenase (LDH) by 47.8% and 69.6%, respectively. These results suggest that the extract has excellent free radical scavenging and anti-oxidative properties.
AuthorsJiang Ping Fan, Chong Fan, Wen Min Dong, Bin Gao, Wei Yuan, Jia Shun Gong
JournalFood and chemical toxicology : an international journal published for the British Industrial Biological Research Association (Food Chem Toxicol) Vol. 59 Pg. 527-33 (Sep 2013) ISSN: 1873-6351 [Electronic] England
PMID23831194 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2013 Elsevier Ltd. All rights reserved.
Chemical References
  • Antioxidants
  • Free Radical Scavengers
  • Pigments, Biological
  • Plant Extracts
  • Solvents
  • Tea
  • Ethanol
  • Malondialdehyde
  • Lactate Dehydrogenases
  • Superoxide Dismutase
Topics
  • Antioxidants (chemistry, isolation & purification, metabolism, pharmacology)
  • Camellia sinensis (chemistry, metabolism)
  • Cell Line
  • Cell Survival (drug effects)
  • China
  • Diet (ethnology)
  • Endothelium, Vascular (cytology, drug effects, enzymology, metabolism)
  • Ethanol (chemistry)
  • Ethnopharmacology
  • Fermentation
  • Food Handling
  • Free Radical Scavengers (adverse effects, chemistry, isolation & purification, pharmacology)
  • Humans
  • Lactate Dehydrogenases (chemistry, metabolism)
  • Malondialdehyde (antagonists & inhibitors, metabolism)
  • Oxidative Stress (drug effects)
  • Pigments, Biological (adverse effects, chemistry, isolation & purification, pharmacology)
  • Plant Extracts (adverse effects, chemistry, isolation & purification, pharmacology)
  • Solubility
  • Solvents (chemistry)
  • Superoxide Dismutase (chemistry, metabolism)
  • Tea (chemistry, microbiology)

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