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[Construction of RNAi vector of dopamine D1 receptor and identification of its silencing effects].

AbstractOBJECTIVE:
To construct dopamine D1 receptor (DRD1) expression interference vectors to study the role of DRD1 in nerve cells and lay a foundation for drug development in anti-convulsion.
METHODS:
Based on DRD1 gene sequence in GenBank, 10 interfere vectors of DRD1 were designed. Liposomal was used to transfect NG-108-15 and the transfect effect was assayed by GFP. With realtime PCR and Western blot, the DRD1 expression was detected.
RESULTS:
The 10 constructed interfere vectors transfected into NG-108-15 cells by liposomal method and inhibited DRD1 mRNA and protein expression. DRD1 mRNA expression in NG-108-15 cells transfected with pGPU6-GFP-Neo-si-DRD1-5 was the lowest whereas DRD1 protein expression in NG-108-15 cells transfected with pGPU6-GFP-Neo-si-DRD1-1, -2, -6, -7 was the lowest.
CONCLUSION:
DRD1 expression interference vector is successfully constructed.
AuthorsHui Li, Junmei Xu, Guixiu Yuan, Jin Li, Zhangling Chen
JournalZhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences (Zhong Nan Da Xue Xue Bao Yi Xue Ban) Vol. 38 Issue 6 Pg. 570-5 (Jun 2013) ISSN: 1672-7347 [Print] China
PMID23828700 (Publication Type: English Abstract, Journal Article)
Chemical References
  • Liposomes
  • RNA, Messenger
  • RNA, Small Interfering
  • Receptors, Dopamine D1
Topics
  • Animals
  • Cell Line, Tumor
  • Genetic Vectors
  • Glioma (pathology)
  • Hybrid Cells
  • Liposomes (metabolism)
  • Mice
  • Neuroblastoma (pathology)
  • RNA Interference
  • RNA, Messenger (genetics, metabolism)
  • RNA, Small Interfering (genetics)
  • Receptors, Dopamine D1 (genetics, metabolism)
  • Transfection

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