Abstract | OBJECTIVE: To construct dopamine D1 receptor (DRD1) expression interference vectors to study the role of DRD1 in nerve cells and lay a foundation for drug development in anti-convulsion. METHODS: Based on DRD1 gene sequence in GenBank, 10 interfere vectors of DRD1 were designed. Liposomal was used to transfect NG-108-15 and the transfect effect was assayed by GFP. With realtime PCR and Western blot, the DRD1 expression was detected. RESULTS: The 10 constructed interfere vectors transfected into NG-108-15 cells by liposomal method and inhibited DRD1 mRNA and protein expression. DRD1 mRNA expression in NG-108-15 cells transfected with pGPU6-GFP-Neo-si-DRD1-5 was the lowest whereas DRD1 protein expression in NG-108-15 cells transfected with pGPU6-GFP-Neo-si-DRD1-1, -2, -6, -7 was the lowest. CONCLUSION: DRD1 expression interference vector is successfully constructed.
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Authors | Hui Li, Junmei Xu, Guixiu Yuan, Jin Li, Zhangling Chen |
Journal | Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences
(Zhong Nan Da Xue Xue Bao Yi Xue Ban)
Vol. 38
Issue 6
Pg. 570-5
(Jun 2013)
ISSN: 1672-7347 [Print] China |
PMID | 23828700
(Publication Type: English Abstract, Journal Article)
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Chemical References |
- Liposomes
- RNA, Messenger
- RNA, Small Interfering
- Receptors, Dopamine D1
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Topics |
- Animals
- Cell Line, Tumor
- Genetic Vectors
- Glioma
(pathology)
- Hybrid Cells
- Liposomes
(metabolism)
- Mice
- Neuroblastoma
(pathology)
- RNA Interference
- RNA, Messenger
(genetics, metabolism)
- RNA, Small Interfering
(genetics)
- Receptors, Dopamine D1
(genetics, metabolism)
- Transfection
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