Expression of DNMTs and genomic DNA methylation in gastric signet ring cell carcinoma.

Abstract	The aim of the present study was to investigate the protein expression of DNA methyltransferases (DNMTs) and genomic DNA methylation status of genomes in gastric signet ring cell carcinoma (SRC). Immunohistochemistry was performed to analyze DNMT expression and methylated DNA immunoprecipitation microarray (MeDIP‑chip) and MeDIP quantitative real‑time PCR (MeDIP‑qPCR) were performed to analyze the genomic DNA methylation status in gastric SRC tissue. An increase in DNMT1 and decrease in DNMT3A expression in SRC tissue was observed compared with matched non‑cancerous tissue. However, expression of other DNMTs, DNMT2, DNMT3B and DNMT3L, was not found to differ significantly between carcinoma and control. The MeDIP‑chip assay revealed that methylation of gene promoters and CpG islands in SRC was higher than those in matched control tissue. However, MeDIP‑qPCR analysis demonstrated that specific tumor‑related genes, including ABL2, FGF18, TRAF2, EGFL7 and RAB33A were aberrantly hypomethylated in SRC tissue. Results of the current study indicate that gastric SRC may produce complex patterns of aberrant DNA methylation and DNMT expression.
Authors	Miao He, Jing Fan, Rong Jiang, Wei-Xue Tang, Zi-Wei Wang
Journal	Molecular medicine reports (Mol Med Rep) Vol. 8 Issue 3 Pg. 942-8 (Sep 2013) ISSN: 1791-3004 [Electronic] Greece
PMID	23820855 (Publication Type: Journal Article)
Chemical References	DNMT3A protein, human DNA Modification Methylases DNMT3L protein, human DNA (Cytosine-5-)-Methyltransferase 1 DNA (Cytosine-5-)-Methyltransferases DNA Methyltransferase 3A DNA methyltransferase 3B DNMT1 protein, human TRDMT1 protein, human
Topics	Aged Carcinoma, Signet Ring Cell (genetics, metabolism, pathology) CpG Islands DNA (Cytosine-5-)-Methyltransferase 1 DNA (Cytosine-5-)-Methyltransferases (genetics, metabolism) DNA Methylation DNA Methyltransferase 3A DNA Modification Methylases (genetics, metabolism) Female Humans Immunoprecipitation Male Middle Aged Oligonucleotide Array Sequence Analysis Promoter Regions, Genetic Real-Time Polymerase Chain Reaction Stomach Neoplasms (genetics, metabolism, pathology)

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