Cytotoxic activity and apoptosis induction by gaillardin.

Abstract

Cytotoxic activity of gaillardin, a sesquiterpene lactone isolated from Inula oculus-christi L. (Asteraceae), was assessed in the human breast adenocarcinoma cell line MCF-7, human hepatocellular carcinoma cell line HepG-2, human non-small cell lung carcinoma cell line A-549, and human colon adenocarcinoma cell line HT-29, resulting in IC50 values of 6.37, 6.20, 4.76, and 1.81 microg/mL, respectively, in the microculture tetrazolium-formazan MTT assay. In vitro apoptosis-inducing properties of gaillardin were also evaluated in MCF-7 cells with the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) assay. The results suggest gaillardin as a candidate for further studies in cancer therapy

• Authors: Maryam Hamzeloo Moghadam, Farzaneh Naghibi, Azadeh Atoofi, Mitra Asgharian Rezaie, Mahboobeh Irani, Mahmoud Mosaddegh
• Journal: Zeitschrift fur Naturforschung. C, Journal of biosciences (Z Naturforsch C J Biosci) 2013 Mar-Apr Vol. 68 Issue 3-4 Pg. 108-12 ISSN: 0939-5075 [Print] Germany
• PMID: 23819305 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Lactones
• Sesquiterpenes
• gaillardin

Topics

• Apoptosis (drug effects)
• Cell Line, Tumor
• Drug Screening Assays, Antitumor
• Humans
• Lactones (pharmacology)
• Sesquiterpenes (pharmacology)