Dietary camphene attenuates hepatic steatosis and insulin resistance in mice.

Abstract

OBJECTIVE:

The aim of this study was to investigate the protective effects of camphene on high-fat diet (HFD)-induced hepatic steatosis and insulin resistance in mice and to elucidate its mechanism of action.

DESIGN AND METHODS:

Male C57BL/6N mice were fed with a normal diet, HFD (20% fat and 1% cholesterol of total diet), or HFD supplemented with 0.2% camphene (CPND) for 10 weeks.

RESULTS:

Camphene alleviated the HFD-induced increases in liver weight and hepatic lipid levels in mice. Camphene also increased circulating adiponectin levels. To examine the direct effects of camphene on adiponectin secretion, 3T3-L1 adipocytes were incubated with camphene. Consistent with in vivo result, camphene increased adiponectin expression and secretion in 3T3-L1 adipocytes. In HFD-fed mice, camphene increased hepatic adiponectin receptor expression and AMP-activated protein kinase (AMPK) activation. Concordant with the activation of adiponectin-AMPK signaling, camphene increased hepatic expression of fatty acid oxidation-related genes and decreased those of lipogenesis-related genes in HFD-fed mice. Moreover, camphene increased insulin-signaling molecules activation and stimulated glucose transporter-2translocation to the plasma membrane in the liver.

CONCLUSIONS:

These results suggest camphene prevents HFD-induced hepatic steatosis and insulin resistance in mice; furthermore, these protective effects are mediated via the activation of adiponectin-AMPK signaling.

• Authors: Sohee Kim, Youngshim Choi, Seoyoon Choi, Yeji Choi, Taesun Park
• Journal: Obesity (Silver Spring, Md.) (Obesity (Silver Spring)) Vol. 22 Issue 2 Pg. 408-17 (Feb 2014) ISSN: 1930-739X [Electronic] United States
• PMID: 23818423 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 The Obesity Society.

Chemical References

• Adiponectin
• Adipoq protein, mouse
• Antioxidants
• Bicyclic Monoterpenes
• Receptors, Adiponectin
• Terpenes
• adiponectin receptor 1, mouse
• adiponectin receptor 2, mouse
• AMP-Activated Protein Kinases
• camphene

Topics

• 3T3-L1 Cells
• AMP-Activated Protein Kinases (chemistry, metabolism)
• Adipocytes, White (metabolism, pathology)
• Adipogenesis
• Adiponectin (agonists, genetics, metabolism)
• Animals
• Antioxidants (metabolism, therapeutic use)
• Bicyclic Monoterpenes
• Dietary Supplements
• Enzyme Activation
• Fatty Liver (metabolism, pathology, physiopathology, prevention & control)
• Gene Expression Regulation
• Insulin Resistance
• Liver (metabolism, pathology, physiopathology)
• Male
• Mice
• Mice, Inbred C57BL
• Organ Size
• Random Allocation
• Receptors, Adiponectin (agonists, genetics, metabolism)
• Signal Transduction
• Terpenes (metabolism, therapeutic use)