Plastic antibody for DNA damage: fluorescent imaging of BPDE-dG adducts in genomic DNA.

Abstract

Exogenous and endogenous genotoxic carcinogens and their in vivo metabolites may result in DNA damage and cause adverse health effects. It is very difficult to specifically detect trace DNA damage in the presence of a large excess of unmodified nucleosides. Here we report a molecularly imprinted polymer (MIP) nanocomposite, namely nanoMIP, which can be used as a "plastic antibody" for specific recognition of benzo[a]pyrene diol epoxide (BPDE)-DNA adduct. Enhanced binding affinity (880 nM) of nanoMIPs was achieved by using two tailor-made functional monomers. The property of binding kinetics was greatly improved in virtue of the well-defined nanostructure, which was fabricated by initiators for continuous activator regeneration-atom transfer radical polymerization (ICAR-ATRP). The BPDE-adducted DNA can be specifically captured by synthetic nanoMIP. By taking advantage of this antibody-mimicking behavior, we further developed a fluorescently imaged particle counting immunoassay (FIPCIA) method for ultrasensitive detection of BPDE-ssDNA adducts using a laser scanning confocal microscope (LSCM). The number of countable fluorescent dots is proportional to the content of BPDE-ssDNA adducts in the DNA sample. The proposed plastic antibody-based FIPCIA method can detect traces of BPDE-DNA adducts as low as 18 pM in human lung carcinoma A549 cells. This highly-sensitive detection of DNA lesions offers a promising alternative to immunogenic antibody-based immunoassays for genomics and DNA modification analysis.

• Authors: Junfa Yin, Zhixin Wang, Maoyong Song, Chao Zhao, Hailin Wang
• Journal: The Analyst (Analyst) Vol. 138 Issue 17 Pg. 4958-66 (Sep 07 2013) ISSN: 1364-5528 [Electronic] England
• PMID: 23817464 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies
• DNA Adducts
• DNA, Single-Stranded
• 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide
• 7,8-dihydroxy-9,10-epoxide-7,8,9,10-tetrahydrobenzo(a)pyrene-10-deoxyguanosine
• Deoxyguanosine

Topics

• 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide (analogs & derivatives, metabolism)
• Antibodies (metabolism)
• Base Sequence
• Biomimetic Materials (chemistry)
• Cell Line, Tumor
• DNA Adducts (metabolism)
• DNA Damage
• DNA, Single-Stranded (chemistry, genetics, metabolism)
• Deoxyguanosine (analogs & derivatives, metabolism)
• Genome, Human (genetics)
• Humans
• Microscopy, Confocal
• Microscopy, Fluorescence
• Molecular Imprinting
• Nanocomposites (chemistry)