Angelicin regulates LPS-induced inflammation via inhibiting MAPK/NF-κB pathways.

Abstract

BACKGROUND:

Angelicin is a furocoumarin found in Psoralea corylifolia L. fruit. The purpose of this study was to investigate the protective ability of angelicin against inflammation in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and LPS-induced in vivo acute lung injury model.

MATERIALS AND METHODS:

The concentrations of tumor necrosis factor alpha (TNF-α) and interleukin (IL)-6 in the culture supernatants of RAW 264.7 cells were determined 24 h after LPS administration. ALI was induced by intratracheal instillation of LPS. Six hours after LPS inhalation, bronchoalveolar lavage fluid and lung tissue samples were obtained for enzyme-linked immunosorbent assay, histologic, and Western blotting analyses.

RESULTS:

The results showed that pretreatment with angelicin markedly downregulated TNF-α and IL-6 levels in vitro and in vivo, and significantly decreased the amount of inflammatory cells, lung wet-to-dry weight ratio, and myeloperoxidase activity in LPS-induced ALI mice. Furthermore, Western blotting analysis results demonstrated that angelicin blocked the phosphorylation of IκBα, NF-κBp65, p38 MAPK, and JNK in LPS-induced ALI.

CONCLUSIONS:

These results suggest that angelicin was potentially advantageous to prevent inflammatory diseases by inhibiting NF-κB and MAPK pathways. Our data indicated that angelicin might be a potential new agent for prevention of inflammatory reactions and diseases in the clinic.

• Authors: Fang Liu, Guo-quan Sun, Hua-yi Gao, Rui-sheng Li, Lanan-Wassy Soromou, Na Chen, Yan-hong Deng, Hai-hua Feng
• Journal: The Journal of surgical research (J Surg Res) Vol. 185 Issue 1 Pg. 300-9 (Nov 2013) ISSN: 1095-8673 [Electronic] United States
• PMID: 23816246 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier Inc. All rights reserved.

Chemical References

• Anti-Inflammatory Agents
• Furocoumarins
• Interleukin-6
• Lipopolysaccharides
• NF-kappa B
• Tumor Necrosis Factor-alpha
• angelicin

Topics

• Acute Lung Injury (chemically induced, drug therapy, immunology)
• Animals
• Anti-Inflammatory Agents (chemistry, pharmacology)
• Cell Line
• Cell Survival (drug effects, immunology)
• Disease Models, Animal
• Furocoumarins (chemistry, pharmacology)
• Interleukin-6 (metabolism)
• Lipopolysaccharides (pharmacology)
• MAP Kinase Signaling System (drug effects, immunology)
• Macrophages (cytology, drug effects, immunology)
• Male
• Mice
• Mice, Inbred BALB C
• NF-kappa B (immunology, metabolism)
• Pneumonia (chemically induced, drug therapy, immunology)
• Tumor Necrosis Factor-alpha (metabolism)