[Mitochondrial mechanisms of apoptosis of human leukemia K562 cells induced by AVVC-1].

Abstract

This study was purpose to investigate apoptosis pathway of leukemia K562 cells induced by anticoagulant fraction from Agkistrodon acutus venom (AVVC-1). The mitochondrial transmembrane potential (ΔΨm) of leukemia K562 cells was detected by flow cytometry with JC-1 single staining. The expression of cytochrome C in the mitochondrial of leukemia K562 cells was analyzed by Western blot after AVVC-1 treatment. The distribution of cytochrome C in leukemia K562 cells was measured by immuno-fluorescence test. The results showed that the potential of mitochondrial membrane decreased after treatment with different concentrations of AVVC-1 (12.5, 25, 50, 100 µg/ml) for 6 h (P < 0.01). The expression level of cytochrome C protein in mitochondria obviously declined after treatment with 30 µg/ml AVVC-1 for 48 h, and the fluorescent intensity of cytochrome C in cytosol was enhanced at the same time. It is concluded that AVVC-1-induced K562 cell apoptosis is related with mitochondrial damage, and cytochrome C may be a useful agent for investigating human leukemia therapy by using AVVC-1.

• Authors: Ru-Qi Zheng, Gen-Bao Zhang, Lu Huang, Kai-Ran Ma, Juan Wu, Shu Li
• Journal: Zhongguo shi yan xue ye xue za zhi (Zhongguo Shi Yan Xue Ye Xue Za Zhi) Vol. 21 Issue 3 Pg. 591-5 (Jun 2013) ISSN: 1009-2137 [Print] China
• PMID: 23815904 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Snake Venoms
• Cytochromes c

Topics

• Agkistrodon
• Animals
• Apoptosis (drug effects)
• Cytochromes c (metabolism)
• Humans
• K562 Cells
• Membrane Potential, Mitochondrial (drug effects)
• Mitochondria (metabolism)
• Snake Venoms (pharmacology)