Abstract |
Augmenter of liver regeneration (ALR) is a crucial factor in the process of proliferation of hepatocytes. Recently, it has been demonstrated that ALR plays an important role of anti-apoptosis in several cell lines, but the biological effects of ALR in acute T lymphoblastic leukemia have remained unclear. In this study, we investigated the effect of ALR on Jurkat T leukemia cell growth and survival. We found that ALR was up-regulated in Jurkat cells and could reduce the sensitivity of Jurkat cells to vincristine, but had a minimal effect on proliferation of Jurkat cells. Results from analysis of flow cytometry showed ALR attenuated apoptotic cells and inhibited G2/M-arrest in vincristine-treated Jurkat cells. Following incubation with ALR, an increase in pro-caspase8, pro-caspase3, pro-PARP and Bcl-2 levels was observed in vincristine-treated Jurkat cells. In summary, the results of this study demonstrate that ALR protects Jurkat T leukemia cells from vincristine-induced cell death via regulation of apoptotic signaling pathways and cell cycle.
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Authors | Yan Shen, Qi Liu, Hang Sun, Xiaofang Li, Na Wang, Hui Guo |
Journal | International immunopharmacology
(Int Immunopharmacol)
Vol. 17
Issue 2
Pg. 162-7
(Oct 2013)
ISSN: 1878-1705 [Electronic] Netherlands |
PMID | 23810409
(Publication Type: Journal Article)
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Copyright | Copyright © 2013 Elsevier B.V. All rights reserved. |
Chemical References |
- Proteins
- Proto-Oncogene Proteins c-bcl-2
- augmenter of liver regeneration factor
- Vincristine
- Parp1 protein, mouse
- Poly (ADP-Ribose) Polymerase-1
- Poly(ADP-ribose) Polymerases
- Caspase 3
- Caspase 8
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Topics |
- Caspase 3
(metabolism)
- Caspase 8
(metabolism)
- Cell Death
(drug effects, immunology)
- Cytoprotection
(drug effects)
- Humans
- Jurkat Cells
- Leukemia, T-Cell
(metabolism)
- Liver Regeneration
- Poly (ADP-Ribose) Polymerase-1
- Poly(ADP-ribose) Polymerases
(metabolism)
- Proteins
(pharmacology, physiology)
- Proto-Oncogene Proteins c-bcl-2
(metabolism)
- Signal Transduction
(drug effects)
- Vincristine
(pharmacology)
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