Abstract | OBJECTIVE: The objective of the present study was to prepare cantharidin-entrapped non-ionic surfactant vesicles (CTD-NSVs) and evaluate their potential in enhancing the antitumor activities and reducing CTD's toxicity. METHODS AND RESULTS: CTD-NSVs were prepared by injection method. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and flow cytometry analysis showed that CTD-NSVs could significantly enhance in vitro toxicity against human breast cancer cell line MCF-7 and induce more significant cell-cycle arrest in G0/G1 phase. Moreover, Hoechst 33342 staining implicated that CTD-NSVs induced higher apoptotic rates in MCF-7 cells than free CTD solution. In vivo therapeutic efficacy was investigated in imprinting control region mice bearing mouse sarcoma S180. Mice treated with 1.0 mg/kg CTD-NSVs showed the most powerful antitumor activity, with an inhibition rate of 52.76%, which was significantly higher than that of cyclophosphamide (35 mg/kg, 40.23%) and the same concentration of free CTD (1.0 mg/kg, 31.05%). In addition, the acute toxicity and liver toxicity of CTD were also distinctly decreased via encapsulating into NSVs. CONCLUSION: Our results revealed that NSVs could be a promising delivery system for enhancing the antitumor activity and simultaneously reducing the toxicity of CTD.
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Authors | Wei Han, Shengpeng Wang, Rixin Liang, Lan Wang, Meiwan Chen, Hui Li, Yitao Wang |
Journal | International journal of nanomedicine
(Int J Nanomedicine)
Vol. 8
Pg. 2187-96
( 2013)
ISSN: 1178-2013 [Electronic] New Zealand |
PMID | 23807847
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- Drug Carriers
- Surface-Active Agents
- Cantharidin
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Topics |
- Analysis of Variance
- Animals
- Antineoplastic Agents
(chemistry, pharmacology, toxicity)
- Apoptosis
(drug effects)
- Cantharidin
(chemistry, pharmacology, toxicity)
- Cell Cycle
(drug effects)
- Cell Survival
(drug effects)
- Drug Carriers
(chemistry, toxicity)
- Humans
- Hydrophobic and Hydrophilic Interactions
- MCF-7 Cells
- Male
- Mice
- Mice, Inbred ICR
- Surface-Active Agents
(chemistry, toxicity)
- Xenograft Model Antitumor Assays
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