Evaluation of the correlation of caspofungin MICs and treatment outcome in murine infections by wild type strains of Candida parapsilosis.

Abstract

We have evaluated the in vitro activity of caspofungin against 36 wild-type strains of Candida parapsilosis sensu stricto using 3 techniques: broth microdilution, disk diffusion, and the determination of minimal fungicidal concentration (MFC). The first 2 methods showed a good in vitro activity of caspofungin, but the MFCs were ≥2 dilutions above their corresponding MICs. In a murine model of disseminated infection, we evaluated the efficacy of caspofungin at 5 mg/kg against 8 strains of C. parapsilosis representing different degrees of in vitro susceptibility (0.12-1 μg/mL). All the isolates responded to treatment and (1→3)-β-D-glucan levels were reduced in all the cases; however, the study revealed differences among isolates, since caspofungin reduced the tissue burden of mice infected with isolates with MICs ≤0.5 μg/mL but was less effective against those with MICs of 1 μg/mL.

• Authors: Valentina Salas, F Javier Pastor, Javier Capilla, Deanna A Sutton, Emilio Mayayo, Annette W Fothergill, Michael G Rinaldi, Josep Guarro
• Journal: Diagnostic microbiology and infectious disease (Diagn Microbiol Infect Dis) Vol. 77 Issue 1 Pg. 41-5 (Sep 2013) ISSN: 1879-0070 [Electronic] United States
• PMID: 23806662 (Publication Type: Journal Article)
• Copyright: Copyright © 2013 Elsevier Inc. All rights reserved.

Chemical References

• Echinocandins
• Lipopeptides
• Proteoglycans
• beta-Glucans
• polysaccharide-K
• Caspofungin

Topics

• Animals
• Candida (drug effects)
• Candidiasis (drug therapy, microbiology)
• Caspofungin
• Colony Count, Microbial
• Disease Models, Animal
• Echinocandins (pharmacology, therapeutic use)
• Lipopeptides
• Male
• Mice
• Microbial Sensitivity Tests
• Proteoglycans
• Treatment Outcome
• beta-Glucans (blood)