Antihyperglycemic effect of fraxetin on hepatic key enzymes of carbohydrate metabolism in streptozotocin-induced diabetic rats.

Abstract

Epidemiological studies have demonstrated that the diabetes mellitus is a serious health burden for both governments and healthcare providers. The present study was hypothesized to evaluate the antihyperglycemic potential of fraxetin by determining the activities of key enzymes of carbohydrate metabolism in streptozotocin (STZ) - induced diabetic rats. Diabetes was induced in male albino Wistar rats by intraperitoneal administration of STZ (40 mg/kg b.w). Fraxetin was administered to diabetic rats intra gastrically at 20, 40, 80 mg/kg b.w for 30 days. The dose 80 mg/kg b.w, significantly reduced the levels of blood glucose and glycosylated hemoglobin (HbA1c) and increased plasma insulin level. The altered activities of the key enzymes of carbohydrate metabolism such as glucokinase, glucose-6-phosphate dehydrogenase, glucose-6-phosphatase, fructose-1,6-bisphosphatase and hepatic enzymes (aspartate transaminase (AST), alanine transaminase (ALT) and alkaline phosphatase (ALP)) in the liver tissues of diabetic rats were significantly reverted to near normal levels by the administration of fraxetin. Further, fraxetin administration to diabetic rats improved body weight and hepatic glycogen content demonstrated its antihyperglycemic potential. The present findings suggest that fraxetin may be useful in the treatment of diabetes even though clinical studies to evaluate this possibility may be warranted.

• Authors: Raju Murali, Subramani Srinivasan, Natarajan Ashokkumar
• Journal: Biochimie (Biochimie) Vol. 95 Issue 10 Pg. 1848-54 (Oct 2013) ISSN: 1638-6183 [Electronic] France
• PMID: 23806420 (Publication Type: Journal Article)
• Copyright: Copyright © 2013 Elsevier Masson SAS. All rights reserved.

Chemical References

• Blood Glucose
• Coumarins
• Glycated Hemoglobin A
• Hypoglycemic Agents
• Insulin
• hemoglobin A1c protein, human
• Streptozocin
• Glycogen
• fraxetin
• Glucosephosphate Dehydrogenase
• Aspartate Aminotransferases
• Alanine Transaminase
• Glucokinase
• Alkaline Phosphatase
• Fructose-Bisphosphatase
• Glucose-6-Phosphatase

Topics

• Alanine Transaminase (metabolism)
• Alkaline Phosphatase (metabolism)
• Animals
• Aspartate Aminotransferases (metabolism)
• Blood Glucose (metabolism)
• Body Weight (drug effects)
• Carbohydrate Metabolism (drug effects)
• Coumarins (pharmacology)
• Diabetes Mellitus, Experimental (chemically induced, drug therapy, enzymology, pathology)
• Drug Administration Schedule
• Fructose-Bisphosphatase (metabolism)
• Glucokinase (metabolism)
• Glucose-6-Phosphatase (metabolism)
• Glucosephosphate Dehydrogenase (metabolism)
• Glycated Hemoglobin (metabolism)
• Glycogen (metabolism)
• Hypoglycemic Agents (pharmacology)
• Insulin (blood)
• Liver (drug effects, enzymology, pathology)
• Male
• Rats
• Rats, Wistar
• Streptozocin