Anti-inflammatory effect of D-allose in cerebral ischemia/reperfusion injury in rats.

Abstract

D-allose, a type of rare sugar, can produce inhibitory effects on activated leukocytes in various organs, including immunosuppressive effects and anti-inflammatory effects, as well as anti-oxyradical effects. The present experiment was performed to investigate the potential anti-inflammatory effects of D-allose in acute cerebral ischemia/reperfusion (I/R) injury. Transient middle cerebral artery occlusion model was applied in rats. D-allose was administered two times via a tail vein (300 mg/kg, 1 hour before ischemia and 10 hours after reperfusion). After 22 hours of reperfusion following 2 hours of ischemia, brain damage was evaluated by cerebral infarct volume. Myeloperoxidase (MPO) activity assay by enzyme-linked immunosorbent assay, and protein expression of MPO and cyclooxygenase-2 (COX-2) by immunohistochemistry were evaluated to investigate the potential mechanisms of D-allose. The experimental results showed that D-allose exhibited significant neuroprotective effects against acute cerebral I/R injury. The infarct volume in D-allose-treated rats (90.9 ± 13.5 mm(3)) was significantly smaller than that in vehicle rats (114.9 ± 15.3 mm(3), p < 0.01). D-allose treatment significantly suppressed the MPO activity and the number of MPO-positive cells compared with those in the vehicle group, suggesting that treatment with D-allose can reduce the infiltration of leukocytes into the ischemic tissue. Treatment of D-allose also significantly decreased the number of COX-2-positive cells and microglial activation in the ischemic tissue. The present results demonstrate that D-allose exerts potent neuroprotective effects against acute cerebral I/R injury, and constitute the first evidence of anti-inflammatory effects of D-allose which considerably contributes to the beneficial effects. Treatment with D-allose might provide a new strategy and clinically beneficial outcome for acute ischemic stroke.

• Authors: Dakuan Gao, Nobuyuki Kawai, Takehiro Nakamura, Feng Lu, Zhou Fei, Takashi Tamiya
• Journal: Neurologia medico-chirurgica (Neurol Med Chir (Tokyo)) Vol. 53 Issue 6 Pg. 365-74 ( 2013) ISSN: 1349-8029 [Electronic] Japan
• PMID: 23803614 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents
• allose
• Peroxidase
• Cyclooxygenase 2
• Glucose

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology)
• Brain (drug effects, pathology)
• Brain Ischemia (pathology)
• Cerebral Infarction (pathology)
• Cyclooxygenase 2 (analysis)
• Enzyme-Linked Immunosorbent Assay
• Glucose (pharmacology)
• Humans
• Male
• Peroxidase (analysis)
• Rats, Sprague-Dawley
• Reperfusion Injury (pathology)