Mushrooms are both food and a source of natural compounds of
biopharmaceutical interest. The purpose of this study was to clarify whether
davallialactone from mushroom extract affected the pathogenesis of
hyperglycemia oxidative stress and the aging process in human diploid fibroblast (HDF) cells. The high-
glucose state with
glucose oxidase resulted in
glucose oxidative stress, induction of inflammatory molecules, dysfunction of
antioxidant molecules, and activation of
mitogen-activated protein kinase (MAPKs) and its downstream signaling in old HDF cells. The exposure of
glucose oxidative stress in middle-stage cells led to stress-induced premature senescence (SIPS) via senescence-associated β-
galactosidase (SA β-gal) activity and displayed replicative senescence phenomena. However,
davallialactone reduces the pathogenesis of
glucose oxidative stress and the aging process through down-regulation of SA β-gal activity. These results strongly suggest that natural compounds, especially mushroom extract
davallialactone, improve the pathogenesis of
glucose oxidative stress and the aging process. Hence,
davallialactone has potential in the treatment of
diabetes mellitus or age-related disease complications.