Abstract |
The purpose of this experiment was to investigate the visible imaging of gastric adenocarcinoma cells in vitro by targeting tumor-associated glycoprotein 72 (TAG-72) with near-infrared quantum dots (QDs). QDs with an emission wavelength of about 550 to 780 nm were conjugated to CC49 monoclonal antibodies against TAG-72, resulting in a probe named as CC49-QDs. A gastric adenocarcinoma cell line (MGC80-3) expressing high levels of TAG-72 was cultured for fluorescence imaging, and a gastric epithelial cell line (GES-1) was used for the negative control group. Transmission electron microscopy indicated that the average diameter of CC49-QDs was 0.2 nm higher compared with that of the primary QDs. Also, fluorescence spectrum analysis indicated that the CC49-QDs did not have different optical properties compared to the primary QDs. Immunohistochemical examination and in vitro fluorescence imaging of the tumors showed that the CC49-QDs probe could bind TAG-72 expressed on MGC80-3 cells.
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Authors | Yun-Peng Zhang, Peng Sun, Xu-Rui Zhang, Wu-Li Yang, Cheng-Shuai Si |
Journal | Nanoscale research letters
(Nanoscale Res Lett)
Vol. 8
Issue 1
Pg. 294
(Jun 22 2013)
ISSN: 1931-7573 [Print] United States |
PMID | 23800369
(Publication Type: Journal Article)
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