Abstract | BACKGROUND: METHODS: 13 healthy canines received CPB with 60 minutes of aortic clamping and cardioplegic arrest, followed by 90 minutes reperfusion. Left ventricular myocardial samples, blood samples and hemodynamic data were taken at different time points. We performed microRNAs microarray experiments upon the left ventricle myocardium tissue of canines before CPB and after reperfusion for 90 minutes by pooling 3 tissue samples together and used qRT-PCR for confirmation. RESULTS: Statistically significant difference was found in mir-499 level before CPB and after reperfusion (T1 vs. T4, p=0.041). We further examined the mir-499 levels by using qRT-PCR in all 13 canines at 4 different time points (T1 vs. T4, p=0.029). Mir-499 expression was negatively correlated with cardiac troponin T (cTnT) and creatine kinase- MB (CK-MB) levels of canines in all time points samples (r=0.469, p<0.001 and r=0.273, p=0.050 respectively). Moreover, higher mir-499 expression level was associated with higher dP/dtmax at 25 minutes and 90 minutes after reperfusion. CONCLUSION:
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Authors | Han Qin, Guang-xian Chen, Meng-ya Liang, Jian Rong, Jian-ping Yao, Hai Liu, Zhong-kai Wu |
Journal | Journal of translational medicine
(J Transl Med)
Vol. 11
Pg. 154
(Jun 21 2013)
ISSN: 1479-5876 [Electronic] England |
PMID | 23800236
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers
- MicroRNAs
- Troponin T
- Creatine Kinase, MB Form
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Topics |
- Animals
- Biomarkers
(metabolism)
- Cardiopulmonary Bypass
- Creatine Kinase, MB Form
(blood)
- Disease Models, Animal
- Dogs
- Female
- Gene Expression Profiling
- Hemodynamics
- Male
- MicroRNAs
(genetics, metabolism)
- Myocardial Reperfusion Injury
(blood, genetics, physiopathology)
- Oligonucleotide Array Sequence Analysis
- Reproducibility of Results
- Time Factors
- Troponin T
(blood)
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