Anti-inflammatory and immunomodulatory activities of stevioside and steviol on colonic epithelial cells.

Abstract

BACKGROUND:

Stevioside is a natural non-caloric sweetener isolated from Stevia rebaudiana Bertoni leaves. We have proposed its effect on attenuation of tumour necrosis factor α (TNF-α) and interleukin 1β (IL-1β) release in lipopolysaccharide (LPS)-stimulated monocytes. In this study, the anti-inflammatory and immunomodulatory activities of stevioside and its metabolite, steviol, on human colon carcinoma cell line (Caco-2) were evaluated.

RESULTS:

Stevioside and steviol, in the doses used in this study, had no cytotoxicity on Caco-2 cells. Anti-inflammatory activities of these two compounds were observed by potentially suppressed LPS-mediated TNF-α, IL-1β and IL-6 release. In addition, stevioside and steviol showed immunomodulatory effects on IκBα activation and nuclear factor kappa B (NF-κB) suppression in western blotting.

CONCLUSION:

Stevioside and steviol attenuate LPS-induced pro-inflammatory cytokine productions by affecting cytokine gene expression via IκBα/NF-κB signalling pathway.

• Authors: Chaiwat Boonkaewwan, Anyanee Burodom
• Journal: Journal of the science of food and agriculture (J Sci Food Agric) Vol. 93 Issue 15 Pg. 3820-5 (Dec 2013) ISSN: 1097-0010 [Electronic] England
• PMID: 23794454 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2013 Society of Chemical Industry.

Chemical References

• Anti-Inflammatory Agents
• Cytokines
• Diterpenes, Kaurane
• Glucosides
• I-kappa B Proteins
• Immunologic Factors
• Inflammation Mediators
• Lipopolysaccharides
• NF-kappa B
• NFKBIA protein, human
• Plant Extracts
• stevioside
• NF-KappaB Inhibitor alpha
• steviol

Topics

• Anti-Inflammatory Agents (pharmacology, therapeutic use)
• Caco-2 Cells
• Colon (drug effects, metabolism)
• Cytokines (metabolism)
• Diterpenes, Kaurane (pharmacology, therapeutic use)
• Epithelial Cells (drug effects, metabolism)
• Gene Expression (drug effects)
• Glucosides (pharmacology, therapeutic use)
• Humans
• I-kappa B Proteins (metabolism)
• Immunologic Factors (pharmacology, therapeutic use)
• Inflammation (chemically induced, drug therapy, metabolism)
• Inflammation Mediators (metabolism)
• Intestinal Mucosa (drug effects, metabolism)
• Lipopolysaccharides
• NF-KappaB Inhibitor alpha
• NF-kappa B (metabolism)
• Phytotherapy
• Plant Extracts (pharmacology, therapeutic use)
• Signal Transduction
• Stevia (chemistry)