Abstract | OBJECTIVES: METHODS: We screened the US Drug Collection Library against metronidazole-susceptible and resistant strains of T vaginalis. Activity was measured by incubating parasites and drugs for 48 h in the presence of tritiated thymidine. Growth inhibition was determined by the reduction of incorporated radioactivity by compounds at 20 μM in comparison to media control. Drugs that showed good initial activity were further tested to calculate IC50 values. Drugs with the most promise were tested together with metronidazole to see if there was any combinatorial effect. RESULTS: Of the 1040 drugs in the library, 83 (8%) reduced growth of a metronidazole-susceptible T vaginalis strain by at least 20%. Of these, IC50 values were calculated for 27 compounds and 8 drugs were evaluated in combination with metronidazole. Disulfiram and nithiamide were non-5-nitroimidazole drugs that showed the best activity against parasites when used alone. Albendazole and coenzyme B12 were the most promising compounds to boost the efficacy of metronidazole. CONCLUSIONS:
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Authors | E Brook Goodhew, W Evan Secor |
Journal | Sexually transmitted infections
(Sex Transm Infect)
Vol. 89
Issue 6
Pg. 479-84
(Sep 2013)
ISSN: 1472-3263 [Electronic] England |
PMID | 23794105
(Publication Type: Journal Article)
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Chemical References |
- Antiprotozoal Agents
- Metronidazole
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Topics |
- Antiprotozoal Agents
(isolation & purification, pharmacology)
- Drug Evaluation, Preclinical
- Drug Resistance
- Humans
- Inhibitory Concentration 50
- Metronidazole
(pharmacology)
- Parasitic Sensitivity Tests
- Trichomonas vaginalis
(drug effects)
- United States
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