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Viral protein-coating of magnetic nanoparticles using simian virus 40 VP1.

Abstract
Artificial beads including magnetite and fluorescence particles are useful to visualize pathologic tissue, such as cancers, from harmless types by magnetic resonance imaging (MRI) or fluorescence imaging. Desirable properties of diagnostic materials include high dispersion in body fluids, and the ability to target specific tissues. Here we report on the development of novel magnetic nanoparticles (MNPs) intended for use as diagnosis and therapy that are coated with viral capsid protein VP1-pentamers of simian virus 40, which are monodispersive in body fluid by conjugating epidermal growth factor (EGF) to VP1. Critically, the coating of MNPs with VP1 facilitated stable dispersion of the MNPs in body fluids. In addition, EGF was conjugated to VP1 coating on MNPs (VP1-MNPs). EGF-conjugated VP1-MNPs were successfully used to target EGF receptor-expressing tumor cells in vitro. Thus, using viral capsid protein VP1 as a coating material would be useful for medical diagnosis and therapy.
AuthorsTeruya Enomoto, Masaaki Kawano, Hajime Fukuda, Wataru Sawada, Takamasa Inoue, Kok Chee Haw, Yoshinori Kita, Satoshi Sakamoto, Yuki Yamaguchi, Takeshi Imai, Mamoru Hatakeyama, Shigeyoshi Saito, Adarsh Sandhu, Masanori Matsui, Ichio Aoki, Hiroshi Handa
JournalJournal of biotechnology (J Biotechnol) Vol. 167 Issue 1 Pg. 8-15 (Aug 10 2013) ISSN: 1873-4863 [Electronic] Netherlands
PMID23791947 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2013 Elsevier B.V. All rights reserved.
Chemical References
  • Capsid Proteins
  • VP1 protein, polyomavirus
  • Citric Acid
  • Epidermal Growth Factor
  • Iron
  • ErbB Receptors
Topics
  • Animals
  • Capsid Proteins (administration & dosage, chemistry)
  • Cell Line, Tumor
  • Citric Acid (chemistry)
  • Epidermal Growth Factor (administration & dosage, chemistry)
  • ErbB Receptors (metabolism)
  • Humans
  • Iron (blood)
  • Magnetic Phenomena
  • Mice
  • Mice, Inbred BALB C
  • Nanoparticles (administration & dosage, chemistry)
  • Serum (chemistry)

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