Detection of promyelocytic leukemia/retinoic acid receptor α (PML/RARα) fusion gene with functionalized graphene oxide.

Abstract

An attempt was made to use functionalized graphene oxide (GO) to detect the Promyelocytic leukemia/Retinoic acid receptor α fusion gene (PML/RARα fusion gene), a marker gene of acute promyelocytic leukemia. The functionalized GO was prepared by chemical exfoliation method, followed by a polyethylene glycol grafting. It is found that the functionalized GO can selectively adsorb the fluorescein isothiocyanate (FITC)-labeled single-stranded DNA probe and quench its fluorescence. The probe can be displaced by the PML/RARα fusion gene to restore the fluorescence, which can be detected by laser confocal microscopy and flow cytometry. These can be used to detect the presence of the PML/RARα fusion gene. This detection method is verified to be fast, simple and reliable.

• Authors: Ran Li, Yanhong Tan, Xiuhua Chen, Fanggang Ren, Yaofang Zhang, Zhifang Xu, Hongwei Wang
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 14 Issue 6 Pg. 12863-72 (Jun 20 2013) ISSN: 1422-0067 [Print] Switzerland
• PMID: 23787474 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Oncogene Proteins, Fusion
• Oxides
• Solutions
• promyelocytic leukemia-retinoic acid receptor alpha fusion oncoprotein
• Graphite
• DNA

Topics

• Adsorption
• Cell Line, Tumor
• DNA (metabolism)
• Flow Cytometry
• Graphite (chemistry)
• Humans
• Microscopy, Confocal
• Oncogene Proteins, Fusion (genetics)
• Oxides (chemistry)
• Solutions
• Spectrometry, Fluorescence