Abstract | BACKGROUND/AIMS: METHOD: To investigate the effect of exercise preconditioning on protecting the kidney from ischemia in Hsp70 deficiency, we measured apoptosis-related factors and Hsps. Hsp70.1 KO and wild-type mice were divided into sham control ( Sham), exercise preconditioning (Ex), renal ischemia-after-exercise (Ex+IR), and ischemia (IR) groups. Where appropriate a treadmill exercise was performed at 20 m/min, 60 min per day on a 0% gradient for 7 days, and renal ischemia was induced by clamping the renal pedicle for 25 min. To characterize the effects of exercise on oxidative stress- and apoptosis-related factors, and Hsp27 and 70 expressions, we performed immunohistochemistry, western blotting and TUNEL assay, and also measured level of serum creatinine. RESULTS: Serum creatinine concentration and 4-HNE expression were raised in the IR group, as were caspases 3, 7 and 9, while Cu- and Mn-SOD levels were reduced, as were those of anti-apoptotic proteins Bcl-XL, Bcl-2 and Hsp27. All these effects were largely reversed by the exercise preconditioning, as was the decrease in apoptotic cells observed after exercise preconditioning. CONCLUSION: Exercise preconditioning has a beneficial effect in inhibiting oxidative stress and apoptotic cell death in the kidney resulting from ischemic injury even under Hsp70 deficiency.
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Authors | Jin Lee, Sok Park, Won Kyu Kim |
Journal | Histology and histopathology
(Histol Histopathol)
Vol. 28
Issue 9
Pg. 1223-33
(09 2013)
ISSN: 1699-5848 [Electronic] Spain |
PMID | 23780557
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- HSP70 Heat-Shock Proteins
- heat-shock protein 70.1
- Creatinine
- Superoxide Dismutase
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Topics |
- Acute Kidney Injury
(metabolism)
- Animals
- Apoptosis
- Creatinine
(blood)
- Gene Expression Regulation
- HSP70 Heat-Shock Proteins
(genetics, metabolism)
- Immunohistochemistry
- In Situ Nick-End Labeling
- Ischemia
(prevention & control)
- Kidney
(metabolism)
- Male
- Mice
- Mice, Knockout
- Oxidative Stress
- Physical Conditioning, Animal
- Superoxide Dismutase
(metabolism)
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