Proteomic-based identification of multiple pathways underlying n-butylidenephthalide-induced apoptosis in LNCaP human prostate cancer cells.

Abstract

Although numerous studies have shown the cancer-preventive properties of butylidenephthalide (BP), there is little report of BP affecting human prostate cancer cells. In the present study, proteomic-based approaches were used to elucidate the anticancer mechanism of BP in LNCaP human prostate cancer cells. BP treatment decreased the viability of LNCaP human prostate cancer cells in a concentration- and time-dependent manner, which was correlated with G0/G1 phase cell cycle arrest. Increased cell cycle arrest was associated with a decrease in the level of CCND1, CDK2, and PCNA proteins and an increase in the level of CDKN2A, CDKN1A, and SFN proteins. Proteomic studies revealed that among 48 differentially expressed proteins, 25 proteins were down-regulated and 23 proteins were up-regulated and these proteins fall into one large protein protein interaction network. Among these proteins, FAS, AIFM1, BIK, CYCS, SFN, PPP2R1A, CALR, HSPA5, DDIT3, and ERN1 are apoptosis and endoplasmic reticulum (ER) stress associated proteins. Proteomic data suggested that multiple signaling pathways including FAS-dependent pathway, mitochondrial pathway, and ER stress pathway are involved in the apoptosis induced by BP.

• Authors: Cheng-Yoong Pang, Sheng-Chun Chiu, Horng-Jyh Harn, Wei-Jun Zhai, Shinn-Zong Lin, Hsueh-Hui Yang
• Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (Food Chem Toxicol) Vol. 59 Pg. 281-8 (Sep 2013) ISSN: 1873-6351 [Electronic] England
• PMID: 23770345 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier Ltd. All rights reserved.

Chemical References

• Antineoplastic Agents, Phytogenic
• Apoptosis Regulatory Proteins
• Cell Cycle Proteins
• Endoplasmic Reticulum Chaperone BiP
• FAS protein, human
• HSPA5 protein, human
• Neoplasm Proteins
• Phthalic Anhydrides
• fas Receptor
• butylidenephthalide

Topics

• Adenocarcinoma (drug therapy, enzymology, metabolism)
• Antineoplastic Agents, Phytogenic (pharmacology)
• Apoptosis (drug effects)
• Apoptosis Regulatory Proteins (agonists, antagonists & inhibitors, genetics, metabolism)
• Cell Cycle Proteins (agonists, antagonists & inhibitors, genetics, metabolism)
• Cell Line, Tumor
• Cell Survival (drug effects)
• Electrophoresis, Gel, Two-Dimensional
• Endoplasmic Reticulum Chaperone BiP
• Endoplasmic Reticulum Stress (drug effects)
• Humans
• Male
• Mitochondria (drug effects, enzymology, metabolism)
• Models, Biological
• Neoplasm Proteins (agonists, antagonists & inhibitors, chemistry, metabolism)
• Peptide Mapping
• Phthalic Anhydrides (pharmacology)
• Prostatic Neoplasms (drug therapy, enzymology, metabolism)
• Resting Phase, Cell Cycle (drug effects)
• Signal Transduction (drug effects)
• fas Receptor (agonists, chemistry, metabolism)