Excessive alcohol consumption can lead to
gastric ulcer and the present work was aimed to examine the protective effect of
tetrahydrocoptisine (
THC) in the model of
ethanol-induced
gastric ulcer in mice. Fasted mice treated with
ethanol 75% (0.5ml/100g) were pre-treated with
THC (10 or 20mg/kg, ip),
cimetidine (100mg/kg, ip) or saline in different experimental sets for a period of 3days, and animals were euthanized 4h after
ethanol ingestion. Gross and microscopic lesions, immunological and biochemical parameters were taken into consideration. The results showed that
ethanol induced gastric damage, improving
nitric oxide (NO) level, increased pro-inflammatory
cytokine (TNF-α and IL-6) levels and
myeloperoxidase (MPO) activity, as well as the expression of nuclear factor-κB (NF-κB) in the
ethanol group. Pretreatment of
THC at doses of 10 and 20mg/kg bodyweight significantly attenuated the gastric lesions as compared to the
ethanol group. These results suggest that the gastroprotective activity of
THC is attributed to reducing NO production and adjusting the pro-inflammatory
cytokine, inhibited neutrophil accumulation and NF-κB expression.