Photodynamic therapeutic activity of indocyanine green entrapped in polymeric nanoparticles.

Abstract

BACKGROUND:

Photodynamic therapy (PDT) is a therapeutic modality involving the use of a photosensitizer agent activated by light of appropriate wavelength to selectively destroy tumor cells. Indocyanine green (ICG) is a promising photosensitive agent for PDT of tumor cells. The main disadvantage of using ICG in PDT is the instability of ICG in aqueous solutions. Encapsulating ICG dye in a biocompatible matrix based on PEBBLE technology showed an improvement of aqueous stability comparing with free ICG dye. The main objective of this study is to investigate the photodynamic effect of ICG-ormosil PEBBLEs on two different cell lines: human breast adenocarcinoma cells (MCF-7) and hepatocellular carcinoma cells (HepG2).

METHODS:

ICG-embedded ormosil PEBBLEs were synthesized based on a sol-gel process, and characterized by transmission electron microscopy and other fluorescence tests. The cell viability was evaluated by MTT and trypan blue assays. Apoptosis, necrosis, and DNA damage (comet assay), were evaluated by fluorescence microscopic tests.

RESULTS:

The results declared that ICG-ormosil PEBBLEs and free ICG both have the same cytotoxic and phototoxic effect on MCF-7 and HepG2 cell lines, where the apoptotic mode of cell death is preferentially occurred in case of PDT using ICG-ormosil PEBBLEs. Both ICG and ICG-ormosil PEBBLEs induced DNA damage after laser exposure. These results would suggest that entrapping ICG in Polymeric nanoparticles forming ICG-ormosil PEBBLEs improve the aqueous stability of the photosensitizer and in the same time retain its photodynamic activity, suggesting that it is preferred to use ICG-ormosil PEBBLEs instead of free ICG dye.

• Authors: Sherien M El-Daly, Amira M Gamal-Eldeen, Mona A M Abo-Zeid, Ibrahim H Borai, Hanaa A Wafay, Abdel-Rahman B Abdel-Ghaffar
• Journal: Photodiagnosis and photodynamic therapy (Photodiagnosis Photodyn Ther) Vol. 10 Issue 2 Pg. 173-85 (May 2013) ISSN: 1873-1597 [Electronic] Netherlands
• PMID: 23769284 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier B.V. All rights reserved.

Chemical References

• Nanocapsules
• Photosensitizing Agents
• Polymers
• Siloxanes
• ormosil
• Indocyanine Green

Topics

• Cell Survival (drug effects, radiation effects)
• Hep G2 Cells
• Humans
• Indocyanine Green (chemistry, therapeutic use)
• MCF-7 Cells
• Nanocapsules (chemistry, therapeutic use)
• Neoplasms, Experimental (drug therapy, pathology)
• Photochemotherapy (methods)
• Photosensitizing Agents (chemistry, therapeutic use)
• Polymers (chemistry)
• Siloxanes (chemistry)
• Treatment Outcome