Intake of
antioxidants from the diet has been recognized to have beneficial health effects, but the potential benefit of taking
antioxidants such as β-
carotene as supplements is controversial. The aim of the present study was to evaluate the potential protective effects of a physiologically relevant concentration (2 μM) of β-
carotene on the
DNA damaging effects of
catechol in mouse
lymphoma L5178Y cells. Two different exposure protocols were used: simultaneous exposure to β-
carotene and
catechol for 3 h; and exposure to
catechol for 3 h after 18 h pre-treatment with the
vitamin. DNA damage was evaluated using the comet assay (employing one procedure for general damage, and another procedure, which also included oxidative DNA damage). Independent of exposure protocol and procedure for comet assay, β-
carotene did not increase the basal level of DNA damage. However, at the highest concentration of
catechol (1 mM), β-
carotene was found to clearly increase the level of
catechol-induced DNA damage, especially in the pre-treated cells. Interestingly, an opposite effect was observed at lower concentrations of
catechol, but the β-
carotene related reduction of
catechol-induced genotoxicity was significant (P < 0.05) only for the procedure including oxidative damage induced by 0.5 mM
catechol. Taken together our results indicate that β-
carotene can both reduce and enhance the
DNA damaging effects of a genotoxic agent such as
catechol. This indicates that it is the level of
catechol-induced DNA damage that seems to determine whether β-
carotene should be regarded as a beneficial or detrimental agent when it comes to its use as a dietary supplement.