In vitro effects of new generation oxazaphosphorines on human promyelocytic leukemia cells.

Abstract

Mafosfamide cyclohexylamine salt (D-17272), 4-hydro-peroxy-cyclophosphamide (D-18864) and glufosfamide (D-19575, beta-D-glucose-isophosphoramide mustard) are new generation oxazaphosphorine agents. The present investigation was undertaken to determine the activity of these three oxazaphosphorines in human promyelocytic leukemia HL-60 cells. The research was conducted using the spectrophotometric MTT assay and the electronic Beckman Coulter and microscopy methods. Functional and morphological changes were observed after exposure of HL-60 cells to the oxazaphosphorine agents. The various patterns of temporary alterations in cell viability, size and count, and also in the frequency of leukemic cells undergoing mitotic catastrophe, apoptosis and necrosis, were shown. Different leukemic cell responses to the action of the three oxazaphosphorines were evaluated. These are the first data comparing the in vitro activity of D-17272, D-18864 and D-19575 against human promyelocytic leukemia cells.

• Authors: Lidia Mazur, Małgorzata Opydo-Chanek, Marta Stojak, Ulf Niemeyer
• Journal: Folia biologica (Folia Biol (Krakow)) Vol. 61 Issue 1-2 Pg. 31-40 ( 2013) ISSN: 0015-5497 [Print] Poland
• PMID: 23767290 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Phosphoramide Mustards

Topics

• Antineoplastic Agents (chemistry, pharmacology)
• Dose-Response Relationship, Drug
• HL-60 Cells
• Humans
• Molecular Structure
• Phosphoramide Mustards (chemistry, pharmacology)