Abstract |
We studied the influence of novel supramolecular substance, [2] rotaxane (TRO-A0001), on caspase signaling and cell viability in cancer cell lines. TRO-A0001 suppressed concentration-dependently cell proliferation. Expression of the cleaved-form caspase-3 and PARP was significantly increased in cells exposed to TRO-A0001. The expression of Bax was increased by TRO-A0001. Furthermore, the down-regulation of Bax by siRNA resulted in growth activation significantly. The morphological analysis demonstrated that TRO-A0001 increased the levels of apoptotic cells in human cancer cell lines. These results suggest that TRO-A0001 induces apoptosis in cancer cells and holds potential as a new anti- tumor medicine.
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Authors | Kazuki Hara, Tatsuya Beppu, Masahiko Kimura, Yoshihiko Fujita, Toshikazu Takata, Kazuto Nishio, Nobufumi Ono |
Journal | Journal of pharmacological sciences
(J Pharmacol Sci)
Vol. 122
Issue 2
Pg. 153-7
( 2013)
ISSN: 1347-8648 [Electronic] Japan |
PMID | 23739594
(Publication Type: Journal Article)
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Chemical References |
- BAX protein, human
- Macromolecular Substances
- Rotaxanes
- bcl-2-Associated X Protein
- Caspases
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Topics |
- Adenocarcinoma
(pathology)
- Apoptosis
(drug effects, genetics)
- Caspases
(metabolism, physiology)
- Colonic Neoplasms
(pathology)
- Humans
- Macromolecular Substances
- Melanoma
(pathology)
- Molecular Targeted Therapy
- Rotaxanes
(pharmacology)
- Signal Transduction
(drug effects, genetics, physiology)
- Tumor Cells, Cultured
- bcl-2-Associated X Protein
(metabolism)
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