Abstract | OBJECTIVE: METHODS: One hundred male Sprague-Dawley (SD) rats were randomly divided into control group (n=10) and paraquat poisoning group (n=90). The animals were sacrificed by exsanguination at 2, 6, 12, 24, 48, 72, 96, 168, 336 hours after administration with 20% parquat solution. The paraffin sections of lung tissue were stained with hematoxylin- eosin (HE) and Masson trichrome to observe the pathological changes. Glucose-regulated protein 78 ( GRP78) was determined by immunohistochemistry, and malondialdehyde (MDA) of lung tissue was measured. The total protein of tissue was abstracted, and the α-smooth muscle actin (α-SMA) and GRP78 was detected by Western blotting. RESULTS: HE and Masson staining demonstrated inflammatory infiltration and collagen deposition in the lung after paraquat administration with a tendency of exaggeration with time, and finally resulted in fibrosis. The expressions of MDA, α-SMA and GRP78 in the lung tissue were significantly increased 2 hours after paraquat administration compared with those of control group (MDA: 1.38 ± 0.18 nmol/mg vs. 0.85 ± 0.05 nmol/mg, α-SMA: 0.23 ± 0.01 vs. 0.14 ± 0.03, GRP78: 0.72 ± 0.02 vs. 0.37 ± 0.06, P<0.05 or P<0.01), and the expressions of MDA and α-SMA were gradually increased with time. GRP78 protein expression was decreased at 72 hours after paraquat administration. CONCLUSIONS:
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Authors | Xiao-Xiao Meng, Kan Liu, Jiu-Ting Tan, Hui Xie, Rui-Lan Wang |
Journal | Zhonghua wei zhong bing ji jiu yi xue
(Zhonghua Wei Zhong Bing Ji Jiu Yi Xue)
Vol. 25
Issue 6
Pg. 331-4
(Jun 2013)
ISSN: 2095-4352 [Print] China |
PMID | 23739565
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Actins
- GRP78 protein, rat
- Heat-Shock Proteins
- smooth muscle actin, rat
- Malondialdehyde
- Paraquat
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Topics |
- Actins
(metabolism)
- Animals
- Endoplasmic Reticulum Stress
- Heat-Shock Proteins
(metabolism)
- Lung
(metabolism, pathology)
- Male
- Malondialdehyde
(metabolism)
- Paraquat
(poisoning)
- Pulmonary Fibrosis
(chemically induced, metabolism, pathology)
- Rats
- Rats, Sprague-Dawley
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