Rhamnetin attenuates melanogenesis by suppressing oxidative stress and pro-inflammatory mediators.

Abstract

Rhamnetin is a naturally occurring polyphenolic compound. In this report, experimental evidence is presented on the suppression of melanogenesis by rhamnetin using B16 murine melanoma cells (B16 cells). To document the underlying anti-melanogenic action of rhamnetin, several key biochemical mediators were quantified: superoxide (O2(•-)), nitric oxide (·NO) and peroxynitrite (ONOO(-)) in vitro, and total reactive species (RS) generation, O2(•-), ·NO and ONOO(-), reduced glutathione (GSH)/GSH-to-oxidized glutathione (GSSG) ratio, prostaglandin E2 (PGE2) and thromboxane B2 (TXB2) in B16 cells. Results revealed that rhamnetin inhibited murine tyrosinase activity, suppressed melanin content and oxidative stress, reducing O2(•-),·NO and ONOO(-) in vitro and total RS generation, O2(•-), ·NO and ONOO(-) in B16 cells, while maintaining a well-balanced GSH/GSSG ratio in B16 cells. Results further revealed that rhamnetin suppressed key pro-inflammatory mediators such as PGE2 and TXB2. Thus, these results strongly indicate that rhamnetin has powerful anti-melanogenic properties through its anti-oxidative and anti-inflammatory actions.

• Authors: You Jung Kim
• Journal: Biological & pharmaceutical bulletin (Biol Pharm Bull) Vol. 36 Issue 8 Pg. 1341-7 ( 2013) ISSN: 1347-5215 [Electronic] Japan
• PMID: 23739488 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• Antioxidants
• Melanins
• Reactive Oxygen Species
• Thromboxane B2
• rhamnetin
• Quercetin
• Monophenol Monooxygenase
• Glutathione
• Dinoprostone
• Glutathione Disulfide

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology)
• Antioxidants (pharmacology)
• Cell Line, Tumor
• Cell Survival (drug effects)
• Dinoprostone (antagonists & inhibitors, metabolism)
• Glutathione (metabolism)
• Glutathione Disulfide (metabolism)
• Melanins (antagonists & inhibitors)
• Melanoma, Experimental (metabolism)
• Mice
• Monophenol Monooxygenase (metabolism)
• Oxidative Stress (drug effects)
• Quercetin (analogs & derivatives, pharmacology)
• Reactive Oxygen Species (metabolism)
• Thromboxane B2 (antagonists & inhibitors, metabolism)