Proteomic analysis of synovial fibroblast-like synoviocytes from rheumatoid arthritis.

Abstract

OBJECTIVES:

The purpose of this study was to identity protein expression patterns of fibroblast-like synoviocytes (FLSs) derived from the synovial tissue of patients with rheumatoid arthritis (RA) and osteoarthritis.

METHODS:

Two-dimensional gel electrophoresis (2-DE) in combination with matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF-MS) was used to visualise and identify differential cellular protein expression profiles in FLSs between RA and control groups. Western-blot analysis was performed to further verify selected differentially-expressed proteins.

RESULTS:

A total of 1633 and 1603 protein spots were examined in synovial FLSs of RA patients and controls, respectively. Ninety-two spots in the RA group were statistically over- or under-expressed compared with controls. Among them, 33 proteins over-expressed by more than 3-fold were then identified by MALDI-TOF-MS analysis. These proteins included enzymatic and structural proteins (e.g. PKM1/M2, α-enolase, ERp60, lamin-A/C), signal transduction proteins (e.g. annexin 11, peroxiredoxin 1, TrpRS), heat-shock/chaperone proteins (e.g. TCP-1, GRP75, HspB5, Bip) and some unknown protein species. Three proteins, namely α-enolase, GRP75 and PKM2, were verified by Western blot and the results were found to be consistent with proteomic analysis.

CONCLUSIONS:

The differentially expressed proteins identified in RA synovial FLSs might be candidate RA-associated proteins and may prove to be promising diagnostic indicators or new therapeutic targets for RA.

• Authors: Xiao-Jun Li, Min Xu, Xiao-Qin Zhao, Jian-Ning Zhao, Fang-Fang Chen, Wei Yu, De-Yu Gao, Bing Luo
• Journal: Clinical and experimental rheumatology (Clin Exp Rheumatol) 2013 Jul-Aug Vol. 31 Issue 4 Pg. 552-8 ISSN: 0392-856X [Print] Italy
• PMID: 23739258 (Publication Type: Journal Article)

Chemical References

• Proteins

Topics

• Aged
• Arthritis, Rheumatoid (metabolism)
• Female
• Fibroblasts (metabolism)
• Humans
• Male
• Middle Aged
• Osteoarthritis (metabolism)
• Proteins (analysis, metabolism)
• Proteomics (methods)
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
• Synovial Membrane (cytology, metabolism)
• Two-Dimensional Difference Gel Electrophoresis