Lung cancer is the leading cause of death among
cancer patients worldwide, and most of them have died from
metastasis. Migration and invasion are prerequisite processes associated with high
metastasis potential in
cancers.
Moscatilin, a bibenzyl derivative isolated from the Thai orchid Dendrobium pulchellum, has been shown to have anticancer effect against numerous
cancer cell lines. However, little is known regarding the effect of
moscatilin on
cancer cell migration and invasion. The present study demonstrates that nontoxic concentrations of
moscatilin were able to inhibit human
nonsmall cell lung cancer H23 cell migration and invasion. The inhibitory effect of
moscatilin was associated with an attenuation of endogenous
reactive oxygen species (ROS), in which
hydroxyl radical (
OH(∙)) was identified as a dominant species in the suppression of filopodia formation. Western blot analysis also revealed that
moscatilin downregulated activated
focal adhesion kinase (phosphorylated FAK, Tyr 397) and activated
ATP-dependent
tyrosine kinase (phosphorylated Akt, Ser 473), whereas their parental counterparts were not detectable changed. In conclusion, our results indicate the novel molecular basis of moscalitin-inhibiting
lung cancer cell motility and invasion and demonstrate a promising antimetastatic potential of such an agent for
lung cancer therapy.