Abstract | AIM: METHOD AND RESULTS: In this study, we generated a recombinant strain of Lb. plantarum to constitutively overexpress B. subtilis oxalate decarboxylase (oxdC) using a host lactate dehydrogenase promoter (PldhL ). The recombinant Lb. plantarum was able to degrade more than 90% oxalate compared to 15% by the wild type. In addition, the recombinant strain also had higher tolerance up to 500 mmol l(-1) oxalate. CONCLUSION: We developed a recombinant Lb. plantarum NC8 that constitutively expressed heterologous oxalate decarboxylase and degraded oxalate efficiently under in vitro conditions. SIGNIFICANCE AND IMPACT OF STUDY: The long-term aim is to develop an efficient strain for future therapy against oxalosis.
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Authors | K Anbazhagan, P Sasikumar, S Gomathi, H P Priya, G S Selvam |
Journal | Journal of applied microbiology
(J Appl Microbiol)
Vol. 115
Issue 3
Pg. 880-7
(Sep 2013)
ISSN: 1365-2672 [Electronic] England |
PMID | 23734819
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2013 The Society for Applied Microbiology. |
Chemical References |
- Oxalates
- Recombinant Proteins
- Carboxy-Lyases
- oxalate decarboxylase
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Topics |
- Carboxy-Lyases
(genetics, metabolism)
- Lactobacillus plantarum
(genetics, growth & development, metabolism)
- Oxalates
(metabolism)
- Recombinant Proteins
(genetics, metabolism)
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