[CD44 regulates epithelial-mesenchymal transition and metastasis in nasopharyngeal cancer cells].

Abstract

OBJECTIVE:

To study the correlation of CD44 with epithelial-mesenchymal transition(EMT) and metastasis in nasopharyngeal cancer cells, and explore the possible mechanism of CD44 regulates EMT and metastasis in nasopharyngeal cancer cells.

METHOD:

The CD44 and EMT-associated proteins in 5-8F and 6-10B nasopharyngeal cancer cell lines were assayed by Western blotting. The erasion trace test was performed to observe the migratory ability of 5-8F and 6-10B nasopharyngeal cancer cells. Using lipid-mediated DNA transfection technique, the low metastatic nasopharyngeal cancer cells 6-10B were transfected in vitro with plasmid which contained CD44 gene, and then new nasopharyngeal cancer cells were obtained. The CD44 and EMT-associated proteins in 6-10B, empty vector transfected and CD44-transfected cells were assayed by Western blotting. The erasion trace test was performed to observe the alteration of migratory ability of nasopharyngeal cancer cells before and after CD44 transfection.

RESULT:

The expression of CD44 and EMT-associated protein MMP-9 in 5-8F was higher than that in 6-10B, but EMT-associated protein E-Cadherin in 5-8F was lower than that in 6-10B. The migratory ability of 5-8F was higher than that of 6-10B. The expression of CD44 and MMP-9 were significantly higher in the CD44-transfected nasopharyngeal cancer cells than in the control groups. Compared with control groups, the migratory ability of CD44-transfected nasopharyngeal cancer cells was significantly increased.

CONCLUSION:

CD44 positively regulates the metastatic ability of nasopharyngeal cancer cells, which is relevant to the process of EMT.

• Authors: Shuang Wang, Shisheng Li, Dinghua Xie, Qinglai Tang, Shuhui Wang, Jiajia Liu, Yuehong Chen, Xinming Yang
• Journal: Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery (Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi) Vol. 27 Issue 5 Pg. 250-4 (Mar 2013) ISSN: 2096-7993 [Print] China
• PMID: 23729111 (Publication Type: Journal Article)

Chemical References

• Hyaluronan Receptors

Topics

• Carcinoma
• Cell Line, Tumor
• Epithelial-Mesenchymal Transition
• Humans
• Hyaluronan Receptors (genetics, metabolism)
• Nasopharyngeal Carcinoma
• Nasopharyngeal Neoplasms (metabolism, pathology)
• Neoplasm Metastasis
• Transfection