Abstract | BACKGROUND: METHODS: Homogenates of porcine thyroids were incubated for 30 min in the presence of nitrobenzene (0.001, 0.01, 0.1, 0.25, 0.5, 1.0, 2.5, 5.0, 7.5, and 10.0 mM). The level of lipid peroxidation products ( malondialdehyde + 4-hydroxyalkenals) was measured spectrophotometrically. Nitrobenzene (7.5 and 10.0 mM) increased lipid peroxidation in the homogenates of porcine thyroids. Subsequently, homogenates of porcine thyroids were incubated for 30 min in the presence of nitrobenzene (7.5 mM) plus one of the antioxidants: melatonin (0.000001, 0.00001, 0.0001, 0.001, 0.01, 0.1, 0.25, 0.5, 1.0, 2.5, 5.0, and 7.5 mM) or PTU (0.01, 0.1, 0.25, 0.5, 1.0, 2.5, 5.0, and 7.5 mM). RESULTS: Lipid peroxidation caused by nitrobenzene was effectively prevented by melatonin, with the lowest effective concentration of 0.0001 mM, being only two orders of magnitude higher than physiological blood concentration in humans. At the same time, PTU revealed protective effects only in the highest used concentration (7.5 mM), which is practically never reached during pharmacological treatment in patients with thyrotoxicosis. CONCLUSIONS:
Melatonin can serve as an effective agent in protection against nitrobenzene-induced lipid peroxidation in porcine thyroid.
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Authors | Krzysztof Zasada, Malgorzata Karbownik-Lewinska |
Journal | Toxicology and industrial health
(Toxicol Ind Health)
Vol. 31
Issue 12
Pg. 1195-201
(Dec 2015)
ISSN: 1477-0393 [Electronic] England |
PMID | 23723263
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © The Author(s) 2012. |
Chemical References |
- Anticarcinogenic Agents
- Antioxidants
- Antithyroid Agents
- Biomarkers
- Carcinogens
- Chromogenic Compounds
- Indoles
- Nitrobenzenes
- Malondialdehyde
- Propylthiouracil
- nitrobenzene
- 1-methyl-2-phenylindole
- Melatonin
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Topics |
- Abattoirs
- Animals
- Anticarcinogenic Agents
(pharmacology)
- Antioxidants
(pharmacology)
- Antithyroid Agents
(pharmacology)
- Biomarkers
(chemistry, metabolism)
- Carcinogens
(antagonists & inhibitors, toxicity)
- Cell-Free System
(drug effects, metabolism)
- Chromogenic Compounds
(chemistry)
- Indoles
(chemistry)
- Lipid Peroxidation
(drug effects)
- Male
- Malondialdehyde
(chemistry, metabolism)
- Melatonin
(pharmacology)
- Nitrobenzenes
(antagonists & inhibitors, toxicity)
- Osmolar Concentration
- Propylthiouracil
(pharmacology)
- Sus scrofa
- Thyroid Gland
(drug effects, metabolism)
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