Abstract | BACKGROUND/AIMS: Pancreatic ductal adenocarcinoma (PDAC) is a highly lethal disease with an increasing incidence worldwide. Due to lack of early diagnosis and poor prognosis, it is rather critical to improve the early diagnosis of PDAC. A comparative proteomic method was used to analyze serum proteins to find a new potential specific marker. METHODS: Comparative analysis of the pancreatic peripheral blood protein profiling from 40 pancreatic cancer patients, 10 pancreatic benign tumor patients, 10 chronic pancreatitis patients and 40 cancer-free controls. The samples were carried out by 2D-differential gel electrophoresis (2D-DIGE) and differentially expressed proteins were identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS). Two up-regulated proteins were further validation by real time RT-PCR, Western blot analysis and Immunohistochemistry (IHC). RESULTS: We identified fourteen differently expressed proteins in PDAC group compared with cancer-free control group, including 9 up-regulation and 5 down-regulation proteins. Increased Complement C3 and alpha-2-HS-glycoprotein (AHSG) were further confirmed by real time RT-PCR, Western blot analysis and IHC. The expressions of Complement C3 and AHSG were higher in PDAC than that in other groups. CONCLUSIONS:
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Authors | Jiong Chen, Wen Wu, Longjiang Chen, Hangcheng Zhou, Renbao Yang, Liwei Hu, Yue Zhao |
Journal | Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.]
(Pancreatology)
2013 May-Jun
Vol. 13
Issue 3
Pg. 290-7
ISSN: 1424-3911 [Electronic] Switzerland |
PMID | 23719603
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2013 IAP and EPC. Published by Elsevier B.V. All rights reserved. |
Chemical References |
- Biomarkers, Tumor
- Complement C3
- alpha-2-HS-Glycoprotein
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Topics |
- Adenocarcinoma
(blood, diagnosis)
- Adult
- Aged
- Aged, 80 and over
- Biomarkers, Tumor
(blood)
- Carcinoma, Pancreatic Ductal
(blood, diagnosis)
- Complement C3
(analysis, biosynthesis)
- Down-Regulation
- Electrophoresis, Gel, Two-Dimensional
(methods)
- Female
- Humans
- Male
- Middle Aged
- Pancreatic Neoplasms
(diagnosis)
- Pancreatitis, Chronic
(blood)
- Proteomics
(methods)
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- Up-Regulation
- alpha-2-HS-Glycoprotein
(analysis, biosynthesis)
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