[Proliferation and apoptosis of choriocarcinoma cell JEG-3 induced by VB2 and its in vitro mechanism].

Abstract

OBJECTIVE:

To investigate the effect of purified vitexin compound 2 (VB2), a noval lignanoid from the acetoacetate extract of Vitex negundo seed on the proliferation and apoptosis as well as the expression of mTOR and 4E-BP1 mRNA signal pathway in human choriocarcinoma JEG-3 cell lines in vitro.

METHODS:

The inhibitory effect of different concentrations of VB2 on JEG-3 cells was examined by methyl thiazolyl tetrazolium (MTT) assay. Flow cytormetry was used to analyze the apoptosis after using different concentrations of VB2, and the expression of mTOR and 4E-BP1 mRNA was determined by RT-PCR.

RESULTS:

The inhibitory rate of JEG-3 cell growth which was cultured with different concentrations of VB2 (2.5, 5.0, 10.0, 20.0, 40.0, 80.0, and 160.0 μmol/L) for 24, 48, or 72 hours increased from (6.34±0.41)% to (85.89±0.81)%, and it was positively correlated with the dose and time of culture (P<0.05). VB2 at 5.0, 10.0, or 20.0 μmol/L increased the rate of JEG-3 cell apoptosis in vitro from (9.26±1.02)% to (35.55±1.24)% after 48 hour culture, which was in a dose dependent manner (P<0.05), while 5.0, 10.0, or 20.0 μmol/L of VB2 down-regulated the mRNA levels of mTOR and 4E-BP1 after 48 hour culture, which presented a significant negative correlation between VB2 and the mRNA levels of mTOR and 4E-BP1(P<0.05).

CONCLUSION:

VB2 can restrain the proliferation of choriocarcinoma cell JEG-3 and induce its apoptosis. This effect may be related to the inhibition of VB2 on the mRNA expression of JEG-3 cell mTOR and 4E-BP1.

• Authors: Jun Deng, Yi Zhang, Zhihui Tan
• Journal: Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences (Zhong Nan Da Xue Xue Bao Yi Xue Ban) Vol. 38 Issue 5 Pg. 476-82 (May 2013) ISSN: 1672-7347 [Print] China
• PMID: 23719531 (Publication Type: Journal Article)

Chemical References

• Adaptor Proteins, Signal Transducing
• Antineoplastic Agents, Phytogenic
• Cell Cycle Proteins
• EIF4EBP1 protein, human
• Phosphoproteins
• Apigenin
• vitexin
• MTOR protein, human
• TOR Serine-Threonine Kinases

Topics

• Adaptor Proteins, Signal Transducing (metabolism)
• Antineoplastic Agents, Phytogenic (chemistry, isolation & purification, pharmacology)
• Apigenin (isolation & purification, pharmacology)
• Apoptosis (drug effects)
• Cell Cycle Proteins
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Choriocarcinoma (pathology)
• Female
• Humans
• Phosphoproteins (metabolism)
• Signal Transduction (drug effects)
• TOR Serine-Threonine Kinases (metabolism)
• Uterine Neoplasms (pathology)
• Vitex (chemistry)