[T-SPOT.TB in the diagnosis of tuberculous peritonitis].

Abstract

OBJECTIVE:

To evaluate the value of T-SPOT.TB in the diagnosis of tuberculous peritonitis (TBP).

METHODS:

A total of 50 patients with clinically suspected TBP admitted from August 2011 to July 2012 from the Third Xiangya Hospital were enrolled in this prospective cohort study. Peripheral blood T-SPOT.TB, purified protein derivatives of tuberculin (PPD) skin test, serum tuberculosis antibody (TB-Ab) and ascitic adenosine deaminase (ADA) were measured in the 50 patients and we compared the diagnostic value of the 4 methods.

RESULTS:

According to the standard of diagnosis of TBP and grouping, 24 patients were diagnosed with TBP, 17 non-TBP and 9 undiagnosed in the end. The sensitivity of T-SPOT.TB for the diagnosis of TBP was 91.7% (22/24), with statistical significance when compared with PPD skin test 37.5% (9/24), serum TB-Ab 16.7% (4/24), and ascitic ADA 36.4% (8/22) (P<0.01). The specificity of T-SPOT.TB for the identification of non-TBP was 76.5% (13/17), without statistical significance when compared with PPD skin test 70.0% (7/10), serum TB-Ab 78.6% (11/14), and ascitic ADA 100% (14/14) (P>0.05). The positive prediction value of T-SPOT.TB for the diagnosis of TBP was 84.6% (22/26), without statistical significance when compared with PPD skin test 75.0% (9/12), serum TB-Ab 57.1% (4/7), and ascitic ADA 100%(8/8) (P>0.05). The negative prediction value of T-SPOT.TB for the identification of non-TBP was 86.7% (13/15), with statistical significance when compared with PPD skin test 31.8% (7/22), serum TB-Ab 35.5% (11/31), and ascitic ADA 50.0% (14/28) (P<0.05).

CONCLUSION:

Peripheral blood T-SPOT.TB for the diagnosis of TBP is highly sensitive, which is better than PPD skin test, serum TB-Ab, and ascitic ADA. T-SPOT.TB has an important reference for diagnosing suspected TBP quickly and accurately.

• Authors: Leliang Zhou, Shourong Shen, Miao He, Xiayu Li, Li Tian
• Journal: Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences (Zhong Nan Da Xue Xue Bao Yi Xue Ban) Vol. 38 Issue 5 Pg. 526-31 (May 2013) ISSN: 1672-7347 [Print] China
• PMID: 23719529 (Publication Type: English Abstract, Journal Article)

Topics

• Adolescent
• Adult
• Aged
• Child
• Enzyme-Linked Immunosorbent Assay (methods)
• Female
• Humans
• Interferon-gamma Release Tests (methods)
• Leukocytes, Mononuclear (immunology)
• Male
• Middle Aged
• Peritonitis, Tuberculous (diagnosis)
• Sensitivity and Specificity
• T-Lymphocytes (immunology)
• Young Adult