The expression of laminin-5 in severe dysplasia/carcinoma in situ and early invasive squamous cell carcinoma: an immunohistochemical study.

Abstract

AIM:

The objective of the present study was to detect the immunohistochemical localization of laminin-5 in the subepithelial basement membrane and to investigate the integrity of the basement membrane in cases of severe dysplasia/carcinoma in situ and early invasive squamous cell carcinoma.

METHODS:

Tissue samples of 55 filed (25-early invasive squamous cell carcinoma; 25-severe dysplasia/carcinoma in situ;5-normal buccal mucosa as controls) cases were collected from the archives of Oral Pathology Department. Routine staining and Immunostaining for laminin-5 (Biogenex) was carried out on the sections. The immunohistochemical localization and integrity of the basement membrane protein isoform laminin-5 was studied in formalin fixed paraffin embedded sections of severe dysplasia/CIS and early invasive squamous cell carcinoma (EISCC). Chi square test was used for statistical analysis. P values <0.05 were considered statistically significant.

RESULTS:

The immunostaining of laminin-5 as observed under light microscope ranged from continuous to complete absence. The laminin-5 staining pattern was more often continuous in severe dysplasia/CIS and discontinuous or absent in EISCC. A statistical comparison between different scores of laminin-5 staining in severe dysplasia/CIS and early invasive squamous cell carcinoma showed a highly significant 'P' value (0.0001).

CONCLUSION:

The status of basal lamina, laminin-5 may provide additional and relevant information about invasive potential of dysplasias. Hence the study of laminin-5 immunohistologically can be regarded as an adjunct to distinguish severe dysplastic lesions from early oral invasive carcinoma.

• Authors: F Rahman, N N Rao, S R Tippu, S Patil, S Agarwal, S Srivastava
• Journal: Minerva stomatologica (Minerva Stomatol) Vol. 62 Issue 5 Pg. 139-46 (May 2013) ISSN: 0026-4970 [Print] Italy
• PMID: 23715200 (Publication Type: Journal Article)

Chemical References

• Biomarkers, Tumor
• Cell Adhesion Molecules
• Neoplasm Proteins
• kalinin

Topics

• Basement Membrane (chemistry, pathology)
• Biomarkers, Tumor (analysis)
• Carcinoma in Situ (chemistry, pathology)
• Carcinoma, Squamous Cell (chemistry, pathology)
• Case-Control Studies
• Cell Adhesion Molecules (analysis)
• Humans
• Mouth Mucosa (chemistry, pathology)
• Mouth Neoplasms (chemistry, pathology)
• Neoplasm Invasiveness
• Neoplasm Proteins (analysis)
• Precancerous Conditions (metabolism, pathology)