Abstract | BACKGROUND: METHODS: 50 adult female TA2 mice were randomly divided into 5 groups including DMSO control, CoCl(2), glibenclamide, CoCl(2) + glibenclamide and paclitaxel. All of these mice were inoculated with TA2 spontaneous breast cancer cells in the left groin. Nine days after inoculation the tumor could be palpated. Different treatments for each group were then subcutaneously administered near the tumors on the 9th and 14th days after injection. Tumor size was measured to determine the growth curve. All mice were sacrificed on the 18th day after initial inoculation and tumor tissues were collected. Some fresh tissues without necrosis were stored at -80°C for mRNA detection and the other tumor tissue was fixed with 10% formalin for H&E and immunohistochemical staining. RESULTS: The growth rate of tumor cells in the CoCl(2) + glibenclamide group was lower than that seen in the other groups. On the 14th day, the average volume of tumor in the CoCl(2) + glibenclamide group was the lowest and the difference has statistical significance (P < 0.05), while the differences among the CoCl(2), glibenclamide and paclitaxel had no statistical significance. The mean percentage of cells expressing MMP9 and PCNA was the lowest in the CoCl(2) + glibenclamide group (P < 0.05). MMP9 mRNA expression paralleled MMP9 protein expression in these groups (P < 0.05). CONCLUSIONS:
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Authors | Zhe Rong, Li Li, Fei Fei, Lailong Luo, Yang Qu |
Journal | Journal of experimental & clinical cancer research : CR
(J Exp Clin Cancer Res)
Vol. 32
Pg. 32
(May 28 2013)
ISSN: 1756-9966 [Electronic] England |
PMID | 23714264
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Proliferating Cell Nuclear Antigen
- RNA, Messenger
- Cobalt
- Matrix Metalloproteinase 9
- cobaltous chloride
- Glyburide
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Topics |
- Animals
- Antineoplastic Combined Chemotherapy Protocols
- Breast Neoplasms
(drug therapy, genetics, pathology)
- Cobalt
(administration & dosage, pharmacology)
- Female
- Gene Expression Regulation, Neoplastic
(drug effects)
- Glyburide
(administration & dosage, pharmacology)
- Humans
- Matrix Metalloproteinase 9
(genetics, metabolism)
- Mice
- Neoplasm Metastasis
- Proliferating Cell Nuclear Antigen
(genetics, metabolism)
- RNA, Messenger
(genetics, metabolism)
- Tumor Burden
(drug effects)
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