HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Enhanced wound vascularization using a dsASCs seeded FPEG scaffold.

Abstract
The bioengineering of autologous vascular networks is of great importance in wound healing. Adipose-derived stem cells (ASCs) are of interest due to their ability to differentiate toward various cell types, including vascular. We hypothesized that adult human ASCs embedded in a three-dimensional PEG-fibrin (FPEG) gel have the ability to modulate vascularization of a healing wound. Initial in vitro characterization of ASCs isolated from discarded burn skin samples (dsASCs) and embedded in FPEG gels indicated they could express such pericyte/smooth muscle cell markers as α-smooth muscle actin, platelet-derived growth factor receptor-β, NG2 proteoglycan, and angiopoietin-1, suggesting that these cells could potentially be involved in a supportive cell role (i.e., pericyte/mural cell) for blood vessels. Using a rat skin excision model, wounds treated with dsASCs-FPEG gels showed earlier collagen deposition and wound remodeling compared to vehicle FPEG treated wounds. Furthermore, the dsASCs-seeded gels increased the number of vessels in the wound per square millimeter by day 16 (~66.7 vs. ~36.9/mm(2)) in these same studies. dsASCs may support this increase in vascularization through their trophic contribution of vascular endothelial growth factor, as determined by in vitro analysis of mRNA and the protein levels. Immunohistochemistry showed that dsASCs were localized to the surrounding regions of large blood-perfused vessels. Human dsASCs may play a supportive role in the formation of vascular structures in the healing wound through direct mechanisms as well as indirect trophic effects. The merging of autologous grafts or bioengineered composites with the host's vasculature is critical, and the use of autologous dsASCs in these procedures may prove to be therapeutic.
AuthorsDavid O Zamora, Shanmugasundaram Natesan, Sandra Becerra, Nicole Wrice, Eunna Chung, Laura J Suggs, Robert J Christy
JournalAngiogenesis (Angiogenesis) Vol. 16 Issue 4 Pg. 745-57 (Oct 2013) ISSN: 1573-7209 [Electronic] Germany
PMID23709171 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)
Chemical References
  • Biomarkers
  • Gels
  • Polyethylene Glycols
  • Fibrinogen
Topics
  • Adult
  • Adult Stem Cells (cytology)
  • Animals
  • Biomarkers
  • Burns (pathology, surgery)
  • Cell Culture Techniques (methods)
  • Cell Differentiation
  • Cells, Cultured
  • Debridement (adverse effects)
  • Extracellular Matrix
  • Fibrinogen
  • Gels
  • Heterografts
  • Humans
  • Male
  • Neovascularization, Physiologic (physiology)
  • Polyethylene Glycols
  • Rats
  • Rats, Nude
  • Regeneration (physiology)
  • Skin (blood supply, injuries)
  • Tissue Scaffolds
  • Transplantation, Autologous
  • Wound Healing (physiology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: