The ameliorative effect of sophoricoside on mast cell-mediated allergic inflammation in vivo and in vitro.

Abstract

Sophoricoside exhibits numerous pharmacological effects, including anti- inflammatory and anti-cancer actions, yet the exact mechanism that accounts for the anti-allergic effects of sophoricoside is not completely understood. The aim of the present study was to elucidate whether and how sophoricoside modulates the mast cell-mediated allergic inflammation in vitro and in vivo. We investigated the pharmacological effects of sophoricoside on both compound 48/80 or histamine-induced scratching behaviors and 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis in mice. Additionally, to find a possible explanation for the anti-inflammatory effects of sophoricoside, we evaluated the effects of sophoricoside on the production of histamine and inflammatory cytokines and activation of nuclear factor-κB (NF-κB) and caspase-1 in phorbol 12-myristate 13-acetate plus calcium ionophore A23187 (PMACI)-stimulated human mast cells (HMC-1). The finding of this study demonstrated that sophoricoside reduced compound 48/80 or histamine-induced scratching behaviors and DNCB-induced atopic dermatitis in mice. Additionally, sophoricoside inhibited the production of inflammatory cytokines as well as the activation of NF-κB and caspase-1 in stimulated HMC-1. Collectively, the findings of this study provide us with novel insights into the pharmacological actions of sophoricoside as a potential molecule for use in the treatment of allergic inflammation diseases.

• Authors: Su-Jin Kim, Gil-Yong Lee, Ji-Wook Jung, Sa-Rang Oh, Eun-Mi Ahn, Sung-Hoon Kim, Seung-Heon Hong, Jae-Young Um
• Journal: Molecules (Basel, Switzerland) (Molecules) Vol. 18 Issue 5 Pg. 6113-27 (May 22 2013) ISSN: 1420-3049 [Electronic] Switzerland
• PMID: 23698058 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• Benzopyrans
• Calcium Ionophores
• Carcinogens
• Cytokines
• Dinitrochlorobenzene
• Irritants
• NF-kappa B
• sophoricoside
• Calcimycin
• Histamine
• Caspase 1
• Tetradecanoylphorbol Acetate

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology)
• Benzopyrans (pharmacology)
• Calcimycin (pharmacology)
• Calcium Ionophores (pharmacology)
• Carcinogens (pharmacology)
• Caspase 1 (metabolism)
• Cell Line
• Cytokines (metabolism)
• Dermatitis, Atopic (chemically induced, drug therapy, metabolism, pathology)
• Dinitrochlorobenzene (adverse effects, pharmacology)
• Histamine (metabolism)
• Humans
• Irritants (adverse effects, pharmacology)
• Male
• Mast Cells (metabolism, pathology)
• Mice
• Mice, Inbred BALB C
• Mice, Inbred ICR
• NF-kappa B (metabolism)
• Tetradecanoylphorbol Acetate (pharmacology)