Decreased levels of cell-division cycle 42 (Cdc42) protein in peripheral lymphocytes from ischaemic stroke patients are associated with Golgi apparatus function.

Abstract

OBJECTIVES:

To investigate levels of cell-division cycle 42 (Cdc42) protein, and their relationship with Golgi apparatus function in peripheral lymphocytes, in patients following ischaemic stroke.

METHODS:

Patients with acute cerebral ischaemic stroke (within 24-72 h of the onset of focal neurological symptoms) and healthy control subjects were enrolled in this prospective case-control study. The cellular location of Cdc42 in peripheral lymphocytes was demonstrated using immunofluorescence. Protein levels of Cdc42 and trans-golgi network protein 2 (TGN46) in peripheral lymphocytes were determined by immunocytochemical staining and Western blotting.

RESULTS:

A total of 38 patients with stroke and 38 control subjects were studied. The mean ± SD percentage of Cdc42-positive lymphocytes from patients with stroke was significantly lower than that in control subjects (39.53 ± 13.55% versus 66.61 ± 23.30%, respectively). Similar findings were demonstrated for TGN46. Cdc42 levels were positively correlated with TGN46 levels (r = 0.92).

CONCLUSIONS:

Acute ischaemic stroke was associated with reduced levels of Cdc42 protein. These findings might lead to the development of drugs that could have therapeutic benefits in patients with acute ischaemic stroke.

• Authors: Xiao-Ye Mo, Ting Li, Zhi-Ping Hu
• Journal: The Journal of international medical research (J Int Med Res) Vol. 41 Issue 3 Pg. 642-53 (Jun 2013) ISSN: 1473-2300 [Electronic] England
• PMID: 23696594 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Membrane Glycoproteins
• TGOLN2 protein, human
• cdc42 GTP-Binding Protein

Topics

• Acute Disease
• Adult
• Aged
• Aged, 80 and over
• Brain Ischemia (diagnosis, genetics, metabolism)
• Case-Control Studies
• Cell Cycle (genetics)
• Female
• Fluorescent Antibody Technique
• Gene Expression
• Golgi Apparatus (genetics, metabolism)
• Humans
• Lymphocytes (metabolism, pathology)
• Male
• Membrane Glycoproteins (genetics, metabolism)
• Middle Aged
• Prospective Studies
• Stroke (diagnosis, genetics, metabolism)
• cdc42 GTP-Binding Protein (genetics, metabolism)