[Effects of class I( phosphatidylinositol-3-kinases inhibitor on gastric cancer cell xenografts in nude mice].

Abstract

OBJECTIVE:

To investigate the effect of recombinant adenovirus (phosphatidylinositol-3-kinases(PI3K)(I()-RNAi-AD which blocks the class I( PI3K signaling pathway on gastric carcinoma cells xenografts in nude mice.

METHODS:

Subcutaneous tumor models of nude mice were established with SGC7901 cells and randomly divided into PI3K(I()-RNAi-AD group, NC-RNAi-GFP-AD group and control group. The tumor size and the inhibitory rate of tumor growth on days 3, 6, and 9 after cell transplantation were measured. The expression of TNF-α, COX2, P53, PCNA, E-cadherin and nm23/DNPK in tumor tissues were detected by immunohistochemistry.

RESULTS:

Tumor growth was significantly inhibited in the PI3K(I()-RNAi-AD group(14.2%, 21.0%, and 28.1%) on days 3, 6, 9 compared with NC-RNAi-GFP-AD group(1.3%, 1.9%, and 2.0%, all P<0.05). The expressions of TNF-α, P53, E-cadherin and nm23/DNPK were up-regulated, and the expressions of COX2 and PCNA were down-regulated in the PI3K(I()-RNAi-AD group by immunohistochemical staining(all P<0.05).

CONCLUSIONS:

PI3K(I()-RNAi-AD can inhibit the growth of SGC7901 cell transplantation tumor in vivo in nude mice by inhibiting cell growth, reducing the capacity of tumor invasion and inhibiting tumor angiogenesis.

• Authors: Ru-Lu Liu, Kui Zhao, Jia-Lei Sun, Li-Yan Yu, Bao-Song Zhu, Xiao-Dong Yang, Chun-Gen Xing
• Journal: Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery (Zhonghua Wei Chang Wai Ke Za Zhi) Vol. 16 Issue 5 Pg. 484-8 (May 2013) ISSN: 1671-0274 [Print] China
• PMID: 23696410 (Publication Type: Journal Article)

Chemical References

• Phosphatidylinositols
• Phosphatidylinositol 3-Kinases
• Class I Phosphatidylinositol 3-Kinases

Topics

• Adenoviridae
• Animals
• Cell Line, Tumor
• Cell Proliferation
• Class I Phosphatidylinositol 3-Kinases
• Heterografts
• Humans
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• Phosphatidylinositol 3-Kinases
• Phosphatidylinositols
• Stomach Neoplasms