Abstract |
Verrucarin A (VA), a protein synthesis inhibitor, derived from the pathogen fungus Myrothecium verrucaria, inhibits growth of leukemia cell lines and activates caspases and apoptosis and inflammatory signaling in macrophages. We have investigated VA-induced growth inhibition in breast cancer cells MDA-MB-231 and T47D and, particularly, the mechanism of VA-induced apoptosis. VA treatment brought about apoptotic cell death in a dose- and time-dependent manner which was associated with chromatin condensation, cell shrinkage, nuclear fragmentation and intracellular ROS production. Mitochondrial membrane depolarization, activation of caspase-3, down-regulation of Bcl-2 expression and up-regulation of Bax and p53 expression were observed. VA thus affects the viability of both the breast cancer cells by triggering ROS-mediated intrinsic mechanism of apoptosis.
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Authors | Kandasamy Palanivel, Veerasamy Kanimozhi, Balamuthu Kadalmani, Mohammad Abdulkader Akbarsha |
Journal | Biotechnology letters
(Biotechnol Lett)
Vol. 35
Issue 9
Pg. 1395-403
(Sep 2013)
ISSN: 1573-6776 [Electronic] Netherlands |
PMID | 23690045
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- Immunologic Factors
- Protein Synthesis Inhibitors
- Trichothecenes
- muconomycin A
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Topics |
- Antineoplastic Agents
(isolation & purification, pharmacology)
- Apoptosis
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Female
- Humans
- Hypocreales
(chemistry)
- Immunologic Factors
(isolation & purification, pharmacology)
- Macrophages
(drug effects)
- Protein Synthesis Inhibitors
(isolation & purification, pharmacology)
- Trichothecenes
(isolation & purification, pharmacology)
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