[Expression pattern of hsa-miR-9 and its association with BCL6 in EBV-positive and EBV-negative Burkitt lymphoma cell lines].

Abstract

OBJECTIVE:

To investigate the differential expression pattern of hsa-miR-9 between EBV-positive and -negative Burkitt lymphoma cell lines and its association with BCL-6.

METHODS:

The expression of hsa-miR9 and BCL-6 mRNAs in EBV(+) Raji and EBV-Ramous cells in mRNA levels were detected using fluorescence quantitative PCR (QRT-PCR). The two cells lines were transiently transfected with hsa-mir9-inhibitor and hsa-mir9-minicsvia Oligofectamine 2000, and the changes in BCL6 expressions was detected using QRT-PCR and Western blotting. Annexin V/PI staining was used to analyze the apoptosis and morphological changes of the transfected cells.

RESULTS:

The expression of Hsa-miR9 and BCL-6 was significantly higher in EBV(+) Raji cells than EBV(-) Ramous cells (P<0.01). BCL-6 mRNA and protein expression was reduced in EBV(+) Raji cells after transfection with hsa-miR9-inhibitor but up-regulated in EBV(-) Ramous cells transfected with hsa-miR9-minics. Flow cytometry revealed a significantly decreased apoptosis rate in EBV(+) Raji cells transfected with hsa-miR9-inhibitor but an increased rate in EBV(-) Ramous cells transfected with hsa-miR9-minics, and the results were confirmed by microscopic observations.

CONCLUSION:

Hsa-miR9 positively regulate the expression of BCL-6 and apoptosis of EBV(+) Raji cells and EBV(-) Ramous cells.

• Authors: Xinzhen Dai, Shaohong Chen, Juan Ge, Xiqun Han, Xinhua Zhou, Ziqin Wu, Tong Zhao
• Journal: Nan fang yi ke da xue xue bao = Journal of Southern Medical University (Nan Fang Yi Ke Da Xue Xue Bao) Vol. 33 Issue 5 Pg. 661-6 (May 2013) ISSN: 1673-4254 [Print] China
• PMID: 23688983 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• BCL6 protein, human
• DNA-Binding Proteins
• MIRN92 microRNA, human
• MicroRNAs
• Proto-Oncogene Proteins c-bcl-6

Topics

• Apoptosis
• Burkitt Lymphoma (genetics, pathology, virology)
• Cell Division
• Cell Line, Tumor
• DNA-Binding Proteins (genetics, metabolism)
• Gene Expression Regulation, Neoplastic
• Herpesvirus 4, Human
• Humans
• MicroRNAs (metabolism)
• Proto-Oncogene Proteins c-bcl-6
• Transfection