Cisplatin has been widely used; however, various disadvantageous side effects afflict patients.
Rikkunshito (RKT), a traditional Japanese herbal medicine, has been widely prescribed in Japan to improve
anorexia; but the mechanisms are unknown. Here we studied whether RKT could improve
anorexia induced by
cisplatin and changes in feeding-regulating
peptides in the hypothalamus in rats. Adult male rats were divided into 4 groups: water+saline (WS), water+cisplatin (WC), RKT+saline (RS), and RKT+cisplatin (RC) groups. Water or RKT (1g/kg) was intragastrically administered for 4 days, from day -1 to day 2, and saline or
cisplatin (6mg/kg) was intraperitoneally (i.p.) administered at day 0. After i.p. administration, cumulative food intake, water intake, urine volume and
body weight were measured. The rats were then decapitated, followed by removal of the brain, and feeding-regulating
peptides in the hypothalamus were measured by in situ hybridization histochemistry. In the three-day measurements, there were no significant changes in cumulative water intake and urine volume. The
body weight and cumulative food intake in WC significantly decreased compared to WS, whereas these were not observed in RC.
Pro-opiomelanocortin (
POMC) and
cocaine and
amphetamine-regulated transcript (CART) in the arcuate nucleus (
ARC) in WC significantly increased, and
neuropeptide Y (NPY) in the
ARC decreased compared to WS, whereas those in RS and RC were comparable to WS. These results suggest that RKT may have therapeutic potential for
anorexia induced by
cisplatin.