The in vivo immunomodulatory and synergistic anti-tumor activity of thymosin α1-thymopentin fusion peptide and its binding to TLR2.

In the present study, the immunomodulatory and synergistic anti-tumor activity of thymosin α1-thymopentin fusion peptide (Tα1-TP5) was investigated in vivo. In addition, the potential receptor of Tα1-TP5 was investigated by surface plasmon resonance (SPR) binding studies. It was found that Tα1-TP5 (305 μg/kg) alleviated immunosuppression induced by hydrocortisone (HC). Tα1-TP5 (305 μg/kg) combined with cyclophosphamide (CY) had a better tumor growth inhibitory effect than CY alone. Furthermore, Tα1-TP5 had a higher affinity (KD=6.84 μmol/L) to toll-like receptor 2 (TLR2) than Tα1 (K(D)=35.4 μmol/L), but its affinity was not significantly different from that of TP5. The results of our present work indicate that Tα1-TP5 can possibly be developed as a new immunomodulatory agent.
AuthorsJuan Li, Yanna Cheng, Xinke Zhang, Lei Zheng, Zhen Han, Pingli Li, Yuliang Xiao, Qian Zhang, Fengshan Wang
JournalCancer letters (Cancer Lett) Vol. 337 Issue 2 Pg. 237-47 (Sep 1 2013) ISSN: 1872-7980 [Electronic] Ireland
PMID23684552 (Publication Type: Journal Article)
CopyrightCopyright © 2013 Elsevier Ireland Ltd. All rights reserved.
Chemical References
  • Antigens, CD86
  • Antineoplastic Agents
  • Antineoplastic Agents, Alkylating
  • Cd86 protein, mouse
  • Histocompatibility Antigens Class I
  • Immunologic Factors
  • Immunosuppressive Agents
  • Tlr2 protein, mouse
  • Toll-Like Receptor 2
  • thymalfasin
  • Thymosin
  • Interferon-gamma
  • Cyclophosphamide
  • Thymopentin
  • Hydrocortisone
  • Animals
  • Antigens, CD86 (metabolism)
  • Antineoplastic Agents (immunology, metabolism, pharmacology)
  • Antineoplastic Agents, Alkylating (pharmacology)
  • Antineoplastic Combined Chemotherapy Protocols (pharmacology)
  • Atrophy
  • Cyclophosphamide (pharmacology)
  • Drug Synergism
  • Histocompatibility Antigens Class I (metabolism)
  • Hydrocortisone (pharmacology)
  • Immunologic Factors (metabolism, pharmacology)
  • Immunosuppressive Agents (pharmacology)
  • Interferon-gamma (blood)
  • Male
  • Melanoma, Experimental (drug therapy, immunology, pathology)
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Protein Binding
  • Skin Neoplasms (drug therapy, immunology, pathology)
  • Surface Plasmon Resonance
  • Thymocytes (drug effects, immunology, pathology)
  • Thymopentin (immunology, metabolism, pharmacology)
  • Thymosin (analogs & derivatives, immunology, metabolism, pharmacology)
  • Thymus Gland (drug effects, immunology, pathology)
  • Time Factors
  • Toll-Like Receptor 2 (metabolism)
  • Tumor Burden (drug effects)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!

Choose Username:
Verify Password: