Transcription analyses of GmICHG, a gene coding for a β-glucosidase that catalyzes the specific hydrolysis of isoflavone conjugates in Glycine max (L.) Merr.

Abstract

Isoflavone conjugate-hydrolyzing β-glucosidase (GmICHG) of soybeans [Glycine max (L.) Merr.] catalyzes the specific hydrolysis of isoflavone conjugates (β-7-O-(malonyl)glucosides of isoflavones) to produce free isoflavones. In this study, changes in the transcription levels of GmICHG in the individual organs of soybean seedlings (cv. Enrei) in response to microbial infection and abiotic stresses were analyzed and compared with those of genes coding for 2-hydroxyisoflavanone synthase (GmIFS) and isoflavone 7-O-glucosyltransferase (GmIF7GT). GmICHG was originally expressed in abundance only in the roots and at low levels only in the other organs. The transcription of GmICHG in the roots and other organs was suppressed upon infection of the roots by Phytophthora sojae. Upon wounding of the cotyledon, a transient long-distance up-regulation of GmICHG transcription in the roots was observed; upon fungal infection in the cotyledon, however, a delayed elevation of GmICHG transcription took place in the roots with the maximum at 10 h after the infection. Such long-distance up-regulation patterns were not observed with either GmIFS or GmIF7GT. The transcription levels of GmICHG remained essentially unchanged upon treatment of the roots with Bradyrhizobium japonicum. The transcription of GmICHG in the roots was also sensitive to a variety of stresses on the roots, such as flooding, elicitation with yeast extract, drought, and treatment with plant hormones such as abscisic, salicylic, and jasmonic acids and ethylene.

• Authors: DongChan Yoo, Takayuki Hara, Naoki Fujita, Toshiyuki Waki, Akio Noguchi, Seiji Takahashi, Toru Nakayama
• Journal: Plant science : an international journal of experimental plant biology (Plant Sci) Vol. 208 Pg. 10-9 (Jul 2013) ISSN: 1873-2259 [Electronic] Ireland
• PMID: 23683924 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Acetates
• Cyclopentanes
• Isoflavones
• Organophosphorus Compounds
• Oxylipins
• Plant Proteins
• methyl jasmonate
• beta-Glucosidase
• ethephon

Topics

• Acetates (pharmacology)
• Biocatalysis (drug effects)
• Biosynthetic Pathways (drug effects, genetics)
• Bradyrhizobium (drug effects, physiology)
• Cotyledon (drug effects, genetics, microbiology)
• Cyclopentanes (pharmacology)
• Gene Expression Profiling
• Gene Expression Regulation, Plant (drug effects)
• Genes, Plant (genetics)
• Hydrolysis (drug effects)
• Isoflavones (chemistry, metabolism)
• Organ Specificity (drug effects, genetics)
• Organophosphorus Compounds (pharmacology)
• Oxylipins (pharmacology)
• Phytophthora (drug effects, physiology)
• Plant Proteins (genetics, metabolism)
• Plant Roots (drug effects, genetics, microbiology)
• Seedlings (drug effects, genetics)
• Soybeans (drug effects, enzymology, genetics, microbiology)
• Stress, Physiological (drug effects, genetics)
• Transcription, Genetic (drug effects)
• beta-Glucosidase (genetics, metabolism)